高脂膳食对SR-AⅠ/Ⅱ基因敲除小鼠白色脂肪组织中UCP2 mRNA表达的影响

目的：观察SR-AⅠ/Ⅱ基因敲除（SR-AⅠ/Ⅱ-/-）小鼠白色脂肪组织中UCP2 mRNA的表达，探讨其与SR-AⅠ/Ⅱ-/-小鼠肥胖易感的关系。方法：应用荧光定量 RT-PCR检测小鼠附睾周白色脂肪组织中UCP2 mRNA的表达；图像分析法评估附睾周脂肪细胞形态学变化；酶法检测甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）血脂水平。结果：经12周高脂饲料喂养，SR-AⅠ/Ⅱ-/-小鼠的体重、血脂（TG、TC、LDL-C）水平、附睾周脂肪垫重量、脂肪细胞面积和直径均大于野生（SR-AⅠ/Ⅱ+/+）对照小鼠（P<0.01）；白色脂肪组织UCP2 mRNA表达量低于SR-AⅠ/Ⅱ+/+小鼠（P<0.01）。结论：SR-AⅠ/Ⅱ-/-小鼠对膳食诱导肥胖（diet-induced obesity, DIO）易感可能与其白色脂肪组织UCP2 mRNA表达异常有关。

Effect of high fat diet on UCP2 mRNA expression in white adipose tissue of SR-A I/II gene knock-out mice

AIM:To observe the relationship between UCP2 mRNA expression in white adipose tissue and diet-induced obesity in SR-A I/II gene knock-out (SR-AⅠ/Ⅱ-/-) mice. METHODS:Fluorescent quantitative RT-PCR was used to detect UCP2 mRNA expression in mice epididymal white adipose tissue. The cellular morphological changes were analyzed by using image analysis. Serum TG, TC and LDL-C concentrations were measured by enzymatic determination. RESULTS:After fed with high fat diet for 12 weeks, average body weight of SR-A I/II-/- mice was much higher than that of wild type (SR-A I/II+/+) control mice (P<0.01), as well as the serum lipid (TG, TC, LDL-C) levels, epididymal fat pad weight, adipocyte area and diameter. UCP2 mRNA expression in white adipose tissue in SR-A I/II-/- mice was significant lower than that in SR-A I/II+/+ control mice (P<0.01). CONCLUSION:Abnormal UCP2 mRNA expression may be associated with SR-A I/II-/- mice susceptive to diet-induced obesity.