Structural proteins of HADEN virus

Purified HADEN virus was disrupted and separated into three polypeptide types in 7.5% neutral sodium dodecyl sulfate-polyacrylamide gels. The major polypeptide (HVP1) had a molecular weight of about 67,000 and accounted for 75–83% of the virion protein. The two minor components (HVP2 and HVP3) had molecular weights of about 77,000 and 85,500, respectively. When HADEN virus polypeptides were compared to the polypeptides of adenovirus-associated virus similar profiles were noted, but the polypeptides of the two viruses were distinguishable when coelectrophoresed on a single gel. Purified low density noninfectious HADEN particles were found to contain three polypeptides with the same molecular weights and concentrations as those found in the infectious virions.