| 原发恶性肿瘤MTS1／p16基因缺失的研究 |
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| 引用本文： | 李淑锋,苏长青,曹祥荣.原发恶性肿瘤MTS1／p16基因缺失的研究[J].临床肿瘤学杂志,1999,4(2):45-47. |
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| 作者姓名： | 李淑锋 苏长青 曹祥荣 |
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| 作者单位： | 南京师范大学生物系(李淑锋!210097南京，曹祥荣!210097南京)，南京八一医院全军肿瘤中心实验科(苏长青!210002) |
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| 摘 要： | 目的:研究MTS1/p16基因在人类恶性肿瘤组织中的变化.方法:采用Southern杂交和多重PCR技术,分析了68例原发肿瘤组织标本,包括非小细胞肺癌31例、食管癌15例、胃癌13例、乳腺癌9例.结果:PCR检测癌旁组织未见p16基因缺失,而不同肿瘤组织标本的p16基因缺失差别很大,总缺失率为13.2%(9/68).Southern杂交检测p16基因的缺失率为17.7%(12/68).结论:MTS1/P16基因缺失在人类恶性肿瘤发生发展中起重要作用,是重要的多肿瘤抑制基因.
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| 关 键 词： | 肿瘤 p16 基因缺失 |
Deletion of MTSl/pl6 gene in human primary cancers |
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| Li Shufeng,Su Changqing,Cao Xiangrong.Deletion of MTSl/pl6 gene in human primary cancers[J].Chinese Clinical Oncology,1999,4(2):45-47. |
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| Authors: | Li Shufeng Su Changqing Cao Xiangrong |
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| Institution: | Li Shufeng,Su Changqing,Cao Xiangrong. Department of Biology,Nanjing Normal University,Nanjing 210097 |
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| Abstract: | Objective: To investigate the alteration of MTSl/pl6 gene in human primary cancers. Method:PCR and Southern blotting were performed on 68 fresh tumor specimens from cancer patients, including 31 lung cancers, 15 esophageal cancers, 13 gastric cancers and 9 breast cancers. Results: The results showed that no deletion was detected in 68 tumor - adjacent tissue samples. However, Diffrerent rate of gene deletion in different tumors was detected by PCR in 68 fresh tumor tissues.Total deletion rate of p16 was 13.2% (9/68) . It was confirmed by Southern blotting analysis with p16 cDNA probe. Conclusion: These date suggest that MTSl/pl6 gene alteration plays a critical role in the development of some cancers. p16 is an important suppressor gene of many tumors. |
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| Keywords: | Malignant tumor p16 gene Gene deletion |
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