| 银染端粒重复序列扩增法检测端粒酶活性及其临床应用 |
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| 引用本文： | 何晓松,俞军,戴美红,刘东利,张传兰,高长明. 银染端粒重复序列扩增法检测端粒酶活性及其临床应用[J]. 临床肿瘤学杂志, 1999, 4(1): 33-35 |
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| 作者姓名： | 何晓松 俞军 戴美红 刘东利 张传兰 高长明 |
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| 作者单位： | 江苏省肿瘤防治研究所 |
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| 基金项目： | 江苏省科委自然科学基金资助项目 |
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| 摘 要： | 目的 建立银染端粒重复序列扩增法(TRAP)测定肿瘤细胞的端粒酶活性.方法:将银染与TRAP相结合,测定了人肿瘤细胞株及穿刺细胞标本的端粒酶活性.结果:人肿瘤细胞株端粒酶活性为阳性,35例病理诊断为恶性肿瘤的穿刺标本中,33例端粒酶活性阳性,12例良性病变均为阴性.结论:银染TRAP法是特异,敏感及快速的端粒酶活性的检测方法,提示该方法可成为恶性肿瘤的诊断指标之一.
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| 关 键 词： | 端粒酶 银染 端粒重复序列扩增 恶性肿瘤 |
Detection of teiomerase activity by silver staining TRAP and its clinic value |
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| He Xiaosong,Yu Jun,Dai Meihong,et al.. Detection of teiomerase activity by silver staining TRAP and its clinic value[J]. Chinese Clinical Oncology, 1999, 4(1): 33-35 |
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| Authors: | He Xiaosong Yu Jun Dai Meihong et al. |
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| Affiliation: | He Xiaosong,Yu Jun,Dai Meihong,et al.Jiangsu institute of cancer research,Nanjing 210009 |
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| Abstract: | Objective: To detect the telomerase activity of human tumor cells by silver staining telomeric repeat amplicatioin protocol (TRAP). Methods: Combining silver stain with TRAP, the telomerase activity of human tumor cells and fine - needle - aspri-ated samples were detected. Remits:The human tumor cells were positive for the telomerase activity. Among 35 cancerous fine - nee-dle- aspriated samples, 33 were positive.Telomerase activity was not detected in 12 negative controls.Conclusions: Silver staining telomeric repeat amplication protocol is specific, sensitive and fast assay for telomerase activity, and it could be one of the marks in cnacer. |
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| Keywords: | Telomerase Silver stain TRAP maliganant tumor |
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