湿热因素对大鼠胃黏膜水通道蛋白3、4基因表达的影响

目的探讨湿热因素对大鼠胃黏膜水通道蛋白（AQP）3、4基因表达的影响。方法30只大鼠随机分成3组，造模20d。对照组正常喂饲；脾虚组隔日喂饲，非喂饲日灌喂番泻叶水煎液；模型组20％蜂蜜水自由饮用，隔日灌服油脂，造模开始后第16～20日每日20时-次日8时将大鼠置入人工气候箱中，造模结束后，采用FQ-PCR方法测定各组胃黏膜AQP3、AQP4基因表达。结果模型大鼠在造模过程中出现湿热证的特征性表现；模型组AQP3、AQP4基因表达水平高于对照组、脾虚组（P〈0．05）。结论通过对大鼠施加内、外湿因素的造模方法可复制出湿热证的证候特征；AQP3的异常表达可能是湿热证的发生机制之一。

Effect of Damp-heat Factor on the Expression of Aquaporin 3, 4 Gene in Gastric Mucosa of Rats

Objective To establish the animal model of damp-heat syndrome （DHS） and explore the expression of aquaporin （AQP） 3, 4 gene in gastric mucosa. Methods Thirty SD rats were randomized into 3 groups and observed for 20 days. Group A （normal group, NG） was fed with routine method. Group B （Pi deficiency syndrome group, PDS） was fed alternatively with routine method and aqua of cassia angustifolia Vah1. Group C （DHS） was fed with fat, honey liquid and treated with damp-heat environment. The expression of AQP 3, 4 gene in the 3 groups were determined by fluorescence quantitative polymerase chain reaction （FQ-PCR）. Results The symptoms and signs in Group C were simiar to the clinical DHS. Expression of AQP3 gene in DHS was statistically significant higher than that in PDS and NG （P〈0.05）. Conclusion The method of feeding fat and honey under damp-heat environment is effective to copy DHS. Abnormal expression of AQP3 gene maybe one of the DHS pathogenesis.