| 红霉素抑制A549细胞中鼻病毒14介导的白介素8和MUC5AC的分泌 |
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| 引用本文： | 吴超民,罗志兵,沈策. 红霉素抑制A549细胞中鼻病毒14介导的白介素8和MUC5AC的分泌[J]. 国际呼吸杂志, 2009, 29(16). DOI: 10.3760/cma.j.issn.1673-436X.2009.016.003 |
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| 作者姓名： | 吴超民 罗志兵 沈策 |
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| 作者单位： | 上海交通大学附属第六人民医院呼吸内科,200233;上海交通大学附属第六人民医院呼吸内科,200233;上海交通大学附属第六人民医院呼吸内科,200233 |
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| 摘 要： | 目的 探讨红霉素是否对鼻病毒14(RV14)介导的细胞因子产生和气道黏液高分泌有抑制作用.方法 EM预处理肺泡Ⅱ型上皮细胞A549 3h后,用RV14刺激细胞,然后采用酶联免疫吸附试验法检测细胞培养上清中细胞因子白介素8和细胞裂解液中黏液蛋白MUC5AC的浓度,并用Western blot检测磷酸化p44/42MAPK信号分子变化情况.结果 红霉索明显抑制RV14介导的白介素8和MUC5AC的产生和分泌,并且对RV14介导的p44/42MAPK的激活也有抑制作用.结论 红霉素可有效抑制RV14介导的细胞因子和黏液蛋白的产生和分泌,并且这种抑制作用可能是通过阻断p44/42MAPK信号分子的激活来实现的.
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| 关 键 词： | 红霉素 鼻病毒 A549细胞 MUC5AC 白介素8 |
Inhibition of erythromycin on rhinovirus-14-induced interleukin-8 and MUC5AC secretion in A549 cells |
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| WU Chao-min,LUO Zhi-bing,SHEN Ce. Inhibition of erythromycin on rhinovirus-14-induced interleukin-8 and MUC5AC secretion in A549 cells[J]. International Journal of Respiration, 2009, 29(16). DOI: 10.3760/cma.j.issn.1673-436X.2009.016.003 |
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| Authors: | WU Chao-min LUO Zhi-bing SHEN Ce |
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| Abstract: | Objective To examine the effect of erythromycin on rhinovirus-14 (RV14)-induced cytokines and airway mucin hypersecretion. Methods A549 cells were pre-incubated with medium containing 10 μmol/L erythromycin for three hours before RV14 infection. Immunoreactive interleukin-8 (IL-8) and MUC5AC were quantitated using dual antibody ELISA kits according to the manufacturer's protocol. And activated p44/42MAPK was tested by Western blot. Results Erythromycin blocked the hypersecretion of IL-8 and MUC5AC protein, and the activation of p44/42MAPK induced by RV14 in the A549 cells.Conclusions Erythromyein may inhibit RV14-indueed IL-8 and MUC5AC hypersecretion by blocking the p44/42MAPK pathway or its upstream regulators. |
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| Keywords: | MUC5AC |
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