Id2和 MMP9的表达及其与食管鳞癌的临床病理特征关系研究

目的：观察细胞分化/DNA 结合抑制因子2(Id2)和基质金属蛋白酶9(MMP9)在食管鳞癌组织中的表达，探讨 Id2和 MMP9表达的意义及相关性。方法应用免疫组织化学方法检测70例食管鳞癌和30例正常食管上皮中 Id2和 MMP9的表达水平。结果食管鳞癌中 Id2和 MMP9的阳性表达率均显著高于正常食管上皮(P <0.01)；Id2和 MMP9的表达水平均随食管鳞癌的浸润加深、临床 TNM 分期级别增高而升高，浸润深度在 T3+T4组的表达水平高于 T1+T2组(P <0.05)，临床Ⅱ期和Ⅲ、Ⅳ期的表达水平高于Ⅰ期(P <0.05)，但 Id2和 MMP9的表达均与食管鳞癌的分化程度无显著相关(P >0.05)。淋巴结转移组中 MMP9的表达水平高于无淋巴结转移组(P <0.05)；而 Id2的表达与淋巴结转移无显著相关(P >0.05)；食管鳞癌组织中 Id2和 MMP9的表达水平呈正相关关系(P <0.05)。结论Id2和 MMP9在食管鳞癌的发生和浸润进展中存在协同促进作用，同时检测对于评估食管鳞癌的生物学行为和预后有重要意义。

Expression of Id2 and MMP9 in esophageal squamous cell carcinoma and its relationship with clinicopathological features

Objective To investigate the expression and clinicopathological significance of inhibitors of differentiation 2(Id2) and Matrix metalloproteinase9(MMP9)in esophageal squamous cell carcinoma(ESCC)and explore their correlation.Methods The expression of Id2 and MMP9 in 70 cases of ESCC and 30 cases of controls were detected by immunohistochemical staining. Results The positive rates of Id2 and MMP9 in ESCC were significantly higher than those in normal esophageal epithelia(P <0.01).Expression levels of Id2 and MMP9 were increased as the infiltration depth and TNM staging increased,and the levels were higher in T3 +T4 group and Ⅱ,Ⅲ,Ⅳ stage than in T1 +T2 group and Ⅰ stage(P <0.05),but not correlate to pathologic grades (P >0.05).Expression level of MMP9 was higher in lymph node metastasis group than in group without lymph node metastasis (P <0.05),but expression levels of Id2 were not related to lymph node metastasis(P >0.05 ).The expression of Id2 and MMP9 were positively correlated(P <0.05).Conclusion Id2 and MMP9 might synergistically promote the tumorigenesis and development of ESCC,and their co-detection can be of great importance for estimation of biological behavior and prognosis of ESCC.