| 二氢杨梅素对胃癌细胞 SGC7901生长活性及凋亡的影响 |
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| 引用本文： | 武彦芳,路玉洁,鞠境.二氢杨梅素对胃癌细胞 SGC7901生长活性及凋亡的影响[J].重庆医学,2015(25). |
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| 作者姓名： | 武彦芳 路玉洁 鞠境 |
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| 作者单位： | 1. 陕西省第四人民医院消化内科,西安,710043;2. 南京医科大学生物化学与分子生物学系 210029 |
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| 摘 要： | 目的：研究藤茶中提取的二氢杨梅素(DMY)对胃癌细胞 SGC7901生长的影响,以筛选高效低毒的抗肿瘤药物。方法使用超声中性水浸提法提取藤茶中的活性成分 DMY,反相高效液相色谱(RP-HPLC)对提取物进行分离纯化,纯度达99%以上,CCK-8比色法检测纯化后的 DMY 对肿瘤细胞存活率的影响；流式细胞术检测 DMY 促进细胞凋亡的能力。结果直接从野生藤茶中提取 DMY,经过 RP-HPLC 分离纯化后的 DMY 的纯度达到99%,DMY 在低浓度时(≤1 mmol)对肿瘤细胞具有显著的杀伤效应(P <0.05),且其抑制率与药物浓度呈剂量-效应关系；同时 DMY 具有促进细胞凋亡的能力,且其促凋亡作用随着浓度的增加而增强。结论藤茶中提取的 DMY 具有显著杀伤胃癌细胞 SGC7901的效应,将来可能成为治疗肿瘤的一类药物。
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| 关 键 词： | 细胞凋亡 胃肿瘤 二氢杨梅素 |
Evaluation of anticancer efficacy of dihydromyricetin in gastric cancer cells SGC7901 |
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| Abstract: | Objective To investigate the anticancer efficacy of dihydromyricetin (DMY)in gastric cancer cells SGC7901. Methods The DMY was puritied using RP-HPLC was almost 98%.The cell viability was assessed by CCK-8 Assay.The cell ap-optosis was assessed by flow cytometry.Using ultrasonic extraction with neutral water and reversed-phase high-performance liquid chromatography,DMY was purified from rattan tea.Results By using RP-HPLC,the purified DMY is almost 99%.DMY inhibi-ted the growth of tumor cells in the low concentration(≤ 1 mmol)with a dose-dependent manner(P <0.05 ).Further study on DMY showed that it induced apoptosis in SGC7901 cell line with a dose-dependent manner.Conclusion DMY is a potential thera-peutic agent for gastric cancer. |
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| Keywords: | apoptosis gastric neoplasms DMY |
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