A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts |
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| Authors: | Seok-Seon Roh Seung-Bae Park Seong-Mo Park Byoung Wook Choi Min-Ho Lee Yul-Lye Hwang Chang Hun Kim Hyun-Ah Jeong Chang Deok Kim Jeung-Hoon Lee |
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| Institution: | Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.;1.Department of Biotechnology, Hanbat National University, Daejeon, Korea.;2.Oriental BioMed Lab, Daejeon, Korea.;3.Oriental Medical Colleage of Daejeon University, Daejeon, Korea. |
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| Abstract: | BackgroundThe fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.ObjectiveThis study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.MethodsBioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.ResultsRasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.ConclusionThese results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture. |
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| Keywords: | Extracellular matrix Fibroblasts Rasatiol |
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