Development and Validation of Reverse-phase High-performance Liquid Chromatography Method for Estimation of Citicoline Sodium in Bulk and Dosage Form |
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Authors: | H. K. MARADIYA VASUNDHARA H. PANSARA |
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Affiliation: | Department of Pharmaceutical Quality Assurance, Veerayatan Institute of Pharmacy, Bhuj-Mandvi Highway, Jakhania-370 460, India;1.Department of Pharmaceutical Quality Assurance, N.R.Vekaria Institute of Pharmacy and Research Centre, C.L. College Campus, Junagadh-362 001, India |
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Abstract: | A simple, accurate, precise and sensitive reverse-phase high-performance liquid chromatography method for the determination of citicoline sodium has been developed and validated. Drug was resolved on a C18 column (Phenomenex Luna, 250×4.6 mm, 10μ), utilizing mobile phase of potassium dihydrogen phosphate buffer and acetonitrile in a ratio of 30:70. Mobile phase was delivered at the flow rate of 1.0 ml/min and detection was carried out at 272 nm. Separation was completed within 2.22 min. Calibration curve was linear with good correlation coefficient (R2=0.999) over a concentration range 10-60 μg/ml. Recovery was between 98.84 and 101.76%. Method was found to be reproducible with relative standard deviation for intra and interday precision of <2.0% over the said concentration range. The method was successfully applied to the determination of the citicoline sodium, it can be very useful and an alternate to performing the stability studies. |
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Keywords: | Acetonitrile citicoline sodium high performance liquid chromatography analysis potassium dihydrogen phosphate |
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