睑板腺癌组织p21、p53蛋白表达及临床意义

目的研究p21、p53蛋白在睑板腺癌组织中表达及临床意义。方法选取80例睑板腺癌患者,取其睑板腺癌组织及其中40例患者的癌旁组织作为检测标本。免疫组化法检测p21、p53蛋白的阳性表达率。睑板腺癌患者睑板腺癌组织p21、p53蛋白表达情况影响因素的多因素分析采用Logistic回归分析。相关性分析采用Spearman相关性分析。结果睑板腺癌组织中p21、p53蛋白阳性表达率均明显高于癌旁组织,差异均有统计学意义(P<0.01)。不同分化程度、淋巴结转移情况、局部浸润情况和复发情况睑板腺癌患者睑板腺癌组织p21、p53蛋白阳性表达率比较,差异均有统计学意义(P<0.05)。淋巴结转移、局部浸润和复发是睑板腺癌患者睑板腺癌组织p21蛋白阳性表达的独立危险因素(P<0.05);淋巴结转移、局部浸润是睑板腺癌患者睑板腺癌组织p 53蛋白阳性表达的独立危险因素(P<0.05)。Spearman相关性分析结果显示,睑板腺癌组织中p21蛋白与p53蛋白表达呈正相关(r=0.413,P<0.01)。结论p21、p53蛋白在睑板腺癌组织表达水平明显较高,其中p21蛋白对早期疾病和预后均存在一定影响,而p53蛋白仅对疾病早期有一定影响,且p21、p53蛋白在睑板腺癌发生、发展过程中具有协同作用。

Expression and clinical significance of p21 and p53 in carcinoma of meibomian gland

Objective To investigate the expression and clinical significance of p21 and p53 protein in carcinoma of meibomian gland.Method Eighty patients with carcinoma of meibomian gland were included in the study,from whom the specimens of tcarcinoma of meibomian gland tissues were collected,besides,surrounding normal tissues were additionally collected from 40 cases among them.The immunohistochemistry assay was adopted to detect the positive expression of p21 and p53 in carcinoma of meibomian gland tissues.Logistic regression analysis was performed to determine the factors involved with the expression of p21 and p53 protein.The correlation was analyzed by Spearman correlation.Result The positive expression rates of p21 and p53 proteins in carcinoma of meibomian gland tissues were significantly higher than those in the surrounding normal tissues,with statistically significant differences observed(P<0.01).There were significant differences in the expression of p21 and p53 protein in different differentiation,lymph node metastasis,local infiltration and recurrence of meibomian adenocarcinoma(P<0.05).Lymph node metastasis,local infiltration and recurrence were independent risk factors for positive p21 expression in the tarsal gland carcinoma of patients(P<0.05);lymph node metastasis and local infiltration were risk factors for p53 expression,correspondingly(P<0.05).Spearman correlation analysis indicated that,p21 and p53 protein in tarsal gland carcinoma tissues were positively correlated between each other(r=0.413,P<0.01).Conclusion p21 and p53 are abnormally highly expressed in carcinoma of meibomian gland tissues,and p21 protein expression is related to the early stage and prognosis of disease,while p53 is only associated with early stage disease,nevertheless,p21 and p53 proteins have synergistic effect on the occurrence and development in carcinoma of meibomian gland.