A comparative study of the antioxidant,antimicrobial, cytotoxic and thrombolytic potential of the fruits and leaves of Spondias dulcis |
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Authors: | Shawkat Md Aminul Islam Kh Tanvir Ahmed Mohammad Kawsar Manik Md Arif Wahid Chowdhury Shafayat Ibne Kamal |
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Institution: | Department of Pharmacy, East West University, Aftabnagar, Dhaka-1212, Bangladesh;Department of Clinical Pharmacy and pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh., Tel: +8801722597925, Fax: 880-2-8615583, E-mail: |
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Abstract: | ObjectiveTo investigate the antioxidant, antimicrobial, cytotoxic and thrombolytic property of the fruits and leaves of Spondias dulcis (S. dulcis).MethodsMethanolic extracts of fruits and leaves of S. dulcis were partitioned with chloroform and dichloromethane. The antioxidant potential of the crude extract and partitioned fractions were evaluated in terms of total phenolic content, total flavonoid content, DPPH radical scavenging potential, reducing potential and total antioxidant capacity by specific standard procedures. The antimicrobial activity was evaluated using disc diffusion method. The cytotoxicity was evaluated by using brine shrimp lethality bioassay and compared with vincristine sulfate. The thrombolytic activity was compared with streptokinase.ResultsThe methanolic fruit extract exhibited the highest phenolic content, flavonoid content and antioxidant capacity, among the other extracts, with the highest DPPH radical scavenging activity at a concentration of 10 µg/mL (IC50: 1.91 µg/mL) and maximum reducing power at a concentration of 100 µg/mL (EC50: 3.58 µg/mL). Though all extract showed moderate antimicrobial activity against the bacterial strains, weak or no activity against fungus. The range of LC50 value of all extracts was 1.335-14.057 µg/mL which was far lower than the cut off index for cytotoxicity. All extracts exhibited statistically significant (P<0.001) thrombolytic activity.ConclusionsOur study suggested that S. dulcis exhibits antimicrobial activities against a wide variety of strains while it possesses significant antioxidant, cytotoxic and thrombolytic activity. |
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Keywords: | Spondias dulcis Antioxidant activity Antimicrobial activity Cytotoxic activity Thrombolytic activity DPPH IC50 LC50 |
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