| 脑缺血再灌流后p53基因表达与细胞凋亡间的关系 |
| |
| 引用本文： | 方瑗,魏桂荣,梅元武,孙圣刚,童萼塘. 脑缺血再灌流后p53基因表达与细胞凋亡间的关系[J]. 华中科技大学学报(医学版), 2001, 30(2): 158-159 |
| |
| 作者姓名： | 方瑗 魏桂荣 梅元武 孙圣刚 童萼塘 |
| |
| 作者单位： | 华中科技大学同济医学院附属协和医院神经科, |
| |
| 摘 要： | 用原位杂交、免疫组化及原位末端标记(TUNEL)的方法观察大鼠大脑中动脉闭塞2h后再通0～72h脑组织p53基因的表达与凋亡细胞的分布.结果发现缺血2h及再灌流1h即可见p53mRNA及其蛋白的表达和凋亡细胞,p53mRNA及其蛋白的表达高峰在缺血再灌流后24h,凋亡细胞数高峰在再灌流24h～48h.提示脑缺血再灌流后诱导p53mRNA及其蛋白的表达和细胞凋亡.
|
| 关 键 词： | p53 细胞凋亡 脑缺血再灌流 |
| 修稿时间： | 2000-02-28 |
Relationship between the Expression of P53 Gene and Apoptosis Following Cerebral Ischemia-Reperfusion |
| |
| Fang Yuan,Wei Guirong,Mei Yuanwu et al. Relationship between the Expression of P53 Gene and Apoptosis Following Cerebral Ischemia-Reperfusion[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2001, 30(2): 158-159 |
| |
| Authors: | Fang Yuan Wei Guirong Mei Yuanwu et al |
| |
| Affiliation: | Fang Yuan,Wei Guirong,Mei Yuanwu et al Department of Neurology,Xiehe Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022 |
| |
| Abstract: | By using in situ hybridization method, immunohistochemistry and TUNEL, P53 mRNA and protein expression was detected and the distribution of apoptosis cells was observed in rat brain after 2 h occlusion of cerebral middle artery followed by 0 to 72 h reperfusion It was found that the expression of P53 mRNA and protein was detectable and apoptic cells could be observed at 1 h reperfusion after ischemia for 2 h P53 mRNA and protein expression reached peak at 24 h and cell apoptosis reached peak at 24 to 48 h of ischemia reperfusion Ischemia reperfusion could induce P53 gene and protein expression and apoptosis of cells |
| |
| Keywords: | p53 apoptosis cerebral ischemia-reperfusion |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
