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| 肌钙蛋白的克隆表达及对卵巢癌SKOV3裸鼠移植瘤的抑制作用 | |
| 引用本文： | 王玉,宿静梅,熊光武,魏于全,王世阆. 肌钙蛋白的克隆表达及对卵巢癌SKOV3裸鼠移植瘤的抑制作用[J]. 现代妇产科进展, 2002, 11(3): 168-170 |
| 作者姓名： | 王玉 宿静梅 熊光武 魏于全 王世阆 |
| 作者单位： | 1. 四川大学华西第二医院妇产科,成都,610010 2. 四川大学华西第一医院肿瘤生物治疗中心 3. 第三军医大学西南医院妇产科 |
| 摘 要： | 目的:研究肌钙蛋白（Troponin,TnI）对卵巢癌的治疗作用。方法:将TnI构建于原核表达载体PTrc99A并转化到大肠杆菌JM109中。细胞在LB培养基中培养到对数生长期后,加IPTG至终浓度为5mmol/L诱导。离心收集菌体,超声破菌。包涵体纯化并复性后在体内、体外抑制血管生成,并用于治疗荷有卵巢癌SKOV3的裸鼠,肿瘤体积每周测2次。结果:TnI能在体内和体外抑制血管生成。TnI治疗组肿瘤生长明显受到抑制,生存率升高。结论:TnI可抑制荷瘤小鼠的卵巢癌生长,延长其生存率。 |
| 关 键 词： | 肌钙蛋白 卵巢肿瘤 新生血管化 |
| 文章编号： | 1004-7973(2002)03-0168-03 |
| 修稿时间： | 2001-11-30 |
Cloning and expression of Troponin and its inhibitory effects on human ovarian carcinoma SKOV3 |
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| Wang Yu ,Su Jingmei ,Xiong Guangwu ,et al.. Cloning and expression of Troponin and its inhibitory effects on human ovarian carcinoma SKOV3[J]. Current Advances In Obstetrics and Gynecology, 2002, 11(3): 168-170 | |
| Authors: | Wang Yu Su Jingmei Xiong Guangwu et al. |
| Affiliation: | Wang Yu 1,Su Jingmei 2,Xiong Guangwu 3,et al. 1. Department of Obstetrics and Gynecol-ogy,Second Hospital of Huaxi Medical School,Sichuan University,Chengdu 610041, 2. Center for Biotherapy of Cancer,the First Hospital of Huaxi Medical School,Sich |
| Abstract: | ve:To explore the potential use of Troponin (TnI) against ovarian carcino-ma . Methods: TnI was expressed from PTrc 99 A Vector in E. coli. JM109. The bacteria were induced in the logarithmic phase with 5mmol/L IPTG. Cells were harvested by centrifugation and disrupted by sonicatin. The insoluble inclusion bodies were purified and renatured. Nude mice with the neo-plasm were treated with TnI systemically. The tumor growth in mice were measured twice per week. Results: TnI was a specific inhibitor of angiogenesis in vivo and in vitro. Intraperitoneal administra-tion of TnI caused partial regression of human ovarian carcinoma SKOV3 growing subcutaneously in nude female mice and improved survival. Conclusion: TnI can inhibit ovarian carcinoma growth in the model of nude mice and increase survival. |
| Keywords: | Troponin Ovarian neoplasms Neovascularization |
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