金属硫蛋白基因多态性与2型糖尿病的关系

目的 :探讨金属硫蛋白 (metallothionein,MT)基因多态性与中国汉族人群中 2型糖尿病 (T2 DM)发病之间的关系。方法 :采用 PCR技术和限制性片段长度多态性 (RFL P)的方法检测 4 1名糖尿病患者和 5 3名正常对照者的金属硫蛋白基因家族中的 MT4基因 (rs6 6 6 6 36 ) 2 0 1 G→ A突变 ,统计各组对象的突变频率。结果 :MT4基因 (rs6 6 6 6 36 ) 2 0 1 G→ A突变型等位基因 (A)频率在糖尿病组和正常对照组中差异有显著性(P<0 .0 5 ,OR=2 .335 ) ;AA,GA和 GG三种基因型频率分布差异也有显著性 (P<0 .0 5 ) ;不同基因型的相对风险分析 ,GA基因型携带者患 2型糖尿病的风险是 GG基因型的 3.4 6 2倍 (OR=3.1 6 2 ,95 % OR CI=1 .389～8.6 2 9)。结论 :MT4突变型等位基因与 2型糖尿病的发生有显著的关联性 ,基因突变使 2型糖尿病的发病风险增加

Association between metallothionein gene polymorphism and type 2 diabetes mellitus

ObjectiveTo detect the association between metallothionein (MT) gene polymorphism and type 2 diabetes mellitus (T2DM) in Chinese Han people MethodsThe techniques of PCR and restriction fragment length polymorphism (RFLP) were used to examine MT4 gene (rs666636) G→A mutation in 41 patients with T2DM and 53 unrelated healthy individuals, and then the mutation frequency was statistically computed ResultsThe alleic frequency (A) of MT4 gene mutation was significantly different between the diabetic and control group ( P <0 05,OR=2 335) The distribution rates of AA, GA and GG had also remarkable differences ( P <0 05) The relative risk of suffering from T2DM of GA genotype was 3 462 times (OR=3 462,95% OR CI=1 389~8 629) GG genotype ConclusionThere′s an association between the alleic gene of MT4 gene mutation and T2DM, and the mutation genotype has increased the risk of suffering from T2DM