rHuIL-2诱导胎儿胸腺细胞中NK和LAK活性水平与胎龄关系的研究

高剂量rHuIL-2可诱导人胸腺细胞分化为具有NK和LAK活性的杀伤细胞。本文对用rHuIL-2诱导人胚胸腺细胞NK和LAK活性与胎龄的关系以及对诱导的杀伤细胞的形态学和表型进行了研究。结果表明:①高剂量rHuIL-2可诱导人胚胸腺细胞分化为具有NK和LAK活性的杀伤细胞。②小于16周龄的胎儿胸腺细胞经过rHuIL-2诱导后,不仅诱导的活化细胞数少,而且NK和LAK功能明显低于24周龄以上的胎儿胸腺细胞;24周以上胎龄的胎儿胸腺细胞经rIL-2诱导后的NK和LAK活性接近新生儿。③rIL-2诱导的人胚胸腺细胞形态学上主要为大颗粒淋巴细胞,其表型为NKH_1~+,CD_1(?)_-的细胞。此结果不仅对于深入了解不同胎龄胸腺绍胞在功能上的个体发育程度提供了客观的指标,而且对于选择适当胎龄胎儿胸腺细胞诱导LAK和NK细胞,作为恶性肿瘤的生物治疗以及探讨外周血NK细胞的来源均有一定的意义。

STUDY ON THE RALATIONSHIP BETWEEN THE HUMAN FETAL AGE AND THE LEVEL OF NK AND LAK CYTOTOXICITY FROM FETAL THYMOCYTES INDUCED BY IL-2

High-dose rHuIL-2 can induce the differentiation of human thymocytes into NK andLAK cells. In this paper,the relationship between the human fetal age and the level of NK and LAK cytotoxicity from fetal thymocytes induced by rHuIL-2 as well as the morphology and phenotypes of IL-2-induced cells were studied. The results showed: (1)high dose rHuIL-2 could induce the differentiation of human fetal thymocytes into NK and LAK cells. (2)there appeared to be an age-related increase in the level of NK and LAK cytotoxicity induced by rHuIL-2. The level of IL2-induced NK and LAK cytotoxicity from fetus less than 16 week old was significantly lower than that from fetus over 24 week;The latter upproximated the level from newborn child. (3) morphologically, IL2-induced thymocytes were chiefly of the large granular lymphocytes (LGL)type. The phenotype of those cells was NKH1+ CD16-. These results were of great significace not only for understanding the ontogenesis of fetal thymus function, but also for selecting suitable fetal thymocytes to produce LAK cells for adoptive immuno-therapy of cancer and for further studies on the origin of peripheral blood NK cells.