| Leptin对人成熟脂肪细胞中NYGGF4基因表达的调控 |
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| 引用本文： | 朱金改,赵亚萍,张春梅,陈小慧,高春林,朱〓春,郭锡熔.Leptin对人成熟脂肪细胞中NYGGF4基因表达的调控[J].南京医科大学学报,2009,29(11):1477-1480. |
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| 作者姓名： | 朱金改 赵亚萍 张春梅 陈小慧 高春林 朱〓春 郭锡熔 |
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| 作者单位： | 南京医科大学附属南京妇幼保健院儿科,江苏 南京〓210004;中国人民解放军第82医院内分泌科,江苏 淮安〓223001;南京医科大学附属南京妇幼保健院儿科,江苏 南京〓210004;南京医科大学附属南京妇幼保健院儿科,江苏 南京〓210004;南京医科大学附属南京妇幼保健院儿科,江苏 南京〓210004;南京医科大学附属南京妇幼保健院儿科,江苏 南京〓210004;南京医科大学儿科医学研究所,江苏 南京〓210029 |
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| 基金项目： | 国家自然科学基金资助（30571978，30973231）；江苏省医学领军人才培养经费（LJ200624） |
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| 摘 要： | 目的:观察leptin对人成熟脂肪细胞中NYGGF4基因表达的调节作用?方法:体外培养人前体脂肪细胞,在诱导人前体脂肪细胞分化成熟的基础上,应用100 ng/ml人重组leptin分别干预成熟脂肪细胞6?24 h,采用实时荧光定量RT-PCR技术检测成熟脂肪细胞中NYGGF4基因mRNA的表达水平?结果:100 ng/ml leptin干预人成熟脂肪细胞6 h后,NYGGF4基因mRNA的相对表达量为0.000 476 ± 0.000 178,显著低于对照组0.001 14 ± 0.000 275,P < 0.05;干预24 h NYGGF4基因mRNA的相对表达量为0.000 835 ± 0.000 211,也显著低于对照组0.001 419 ± 0.000 193,P < 0.05?结论:leptin能显著下调成熟脂肪细胞中NYGGF4基因的表达?
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| 关 键 词： | 人前体脂肪细胞 诱导分化 NYGGF4基因 leptin |
| 收稿时间： | 2009/4/24 0:00:00 |
Regulative role of leptin on NYGGF4 gene expression in matured human adipocytes |
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| ZHU Jin-gai,ZHAO Ya-ping,ZHANG Chun-mei,CHEN Xiao-hui,GAO Chun-lin,ZHU Chun and GUO Xi-rong.Regulative role of leptin on NYGGF4 gene expression in matured human adipocytes[J].Acta Universitatis Medicinalis Nanjing,2009,29(11):1477-1480. |
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| Authors: | ZHU Jin-gai ZHAO Ya-ping ZHANG Chun-mei CHEN Xiao-hui GAO Chun-lin ZHU Chun and GUO Xi-rong |
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| Abstract: | Objective:To explore the regulative role of leptin on NYGGF4 gene expression in matured human adipocytes. Methods:Human preadipocytes were cultured in vitro and differentiated into matured adipocytes. The matured adipocytes were treated with 100 ng/ml leptin for 6 hours and 24 hours,respectively. Total RNA from these adipocytes was extracted and the levels of NYGGF4 mRNA expression were evaluated by real-time quantitative RT-PCR. Results:Treatment with 100 ng/ml leptin for 6 hours or 24 hours significantly decreased NYGGF4 gene mRNA expression in human matured adipocytes (P < 0.05). Conclusion:Leptin could downregulate the expression of NYGGF4 gene mRNA in human matured adipocytes. |
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| Keywords: | human preadipocyte cell differentiation NYGGF4 gene leptin |
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