HLA-A等位基因与急发型自身免疫性糖尿病遗传易感性关系的研究

目的:研究江苏地区HLA-A基因型与急发型自身免疫性糖尿病的相关性?方法:选取江苏地区64例自发酮症急性起病?依赖胰岛素治疗的糖尿病患者,从外周血中提取细胞基因组DNA,利用聚合酶链反应-寡核苷酸探针序列特异性引物(PCR-SSO)技术,进行HLA-A的分型,以直接计数法计算等位基因频率?结果:在64例1型糖尿病患者中检出的HLA-A等位基因种类与健康对照组HLA-A基因种类无显著差别(P > 0.05),部分等位基因频率存在显著差异:糖尿病组与健康对照组相比,HLA-A*03(7.82% vs 2.43%,P < 0.05);A*24(26.04% vs 16.78%,P < 0.05);A*01(1.72 % vs 3.07%,P < 0.05);A*11(15.67% vs 21.7%,P < 0.05);HLA-A*31(0.1% vs 3.7%,P < 0.05)?结论:HLA-A*03和A*24在急发型自身免疫性糖尿病患者中均呈现有统计学意义的升高,可能对急发型自身免疫性糖尿病存在易感性;HLA-A*01?A*11和A*31呈现有统计学意义的降低,可能存在保护作用?

Relationship between HLA-A alleles and acute-onset autoimmune diabetes

Objective:To explore the relationship between HLA-A alleles and acute-onset autoimmune diabetes in Jiangsu area. Methods:64 patients with acute-onset ketotic and insuln-depdent diabetic in Jiangsu area were recruited. Peripheral blood cell genomic DNA was extracted from,and detected using polymerase chain reaction restriction sequence specific oligonacleotide(PCR-SSO) technology,and the HLA-A alleles frequencies were studied. Results:The sorts of HLA-A alleles are not different between acute autoimmune diabetes group and healthy control group. The frenquencies of HLA-A*03 and HLA-A*24 alleles in the patients were significantly higher than that in healthy control group(P < 0.05). The frenquencies of HLA-A*01,HLA-A*11,HLA-A*31 alleles in the patients with acute autoimmune diabetes were significantly lower than those in the healthy control group(P < 0.05). Conclusion:HLA-A*03 and HLA-A*24 alleles might be susceptible genes of acute autoimmune diabetes in Jiangsu area. A*01?HLA-A*11?HLA-A*31 might be protective genes.