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Detection of enterovirus genome sequence from diarrheal feces of goat |
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| Authors: | Tsutomu Omatsu Shinobu Tsuchiaka Teppei Hirata Yasushi Shiroma Sachiko Okazaki Yukie Katayama Mami Oba Naomi Nishiura Yukiko Sassa Tetsuya Furuya Makoto Nagai Hideharu Ochiai Shirou Tamaki Tetsuya Mizutani |
| Institution: | 1. Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan 2. The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu, Gifu, 501-1193, Japan 3. Department of Information Engineering, University of the Ryukyus, Nishihara, Nakagami, Okinawa, 903-0213, Japan 4. Department of Electrical and Electronic Engineering, University of the Ryukyus, Nishihara, Nakagami, Okinawa, 903-0213, Japan 5. Laboratory of Microbiology, Department of Veterinary Medicine Faculty and Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan 6. Laboratory of Epizootiology, Department of Veterinary Medicine Faculty and Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan 7. Research Institute of Biosciences, Azabu University, School of Veterinary Medicine, 1-17-71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa, 252-5201, Japan |
| Abstract: | Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5′-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats. |
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