多孔支架材料PLGA/HA生物相容性的实验研究

目的:探讨多孔支架材料聚乳酸羟基乙酸/羟基磷灰石(poly lactic-glycolic acid/hydroxyapatite,PLGA/HA)的生物相容性。方法:贴壁法培养兔骨髓基质细胞(bone marrow stromal cells,BMSCs),经体外诱导、扩增,采用实验组A:PLGA/HA(5%HA)、实验组B:PLGA/HA(10%HA)的梯度浸提液进行体外培养,应用MTT法评估材料的细胞毒性;BMSCs与实验组A、实验组B、对照组C(polylactic-glycolicacid,PLGA)进行体外复合培养,通过定性及定量检测细胞在材料表面的粘附、增殖能力及成骨活性,比较分析各组支架材料之间的差异。结果:两实验组材料的细胞毒性均为0或1级;BMSCs能在实验组和对照组支架材料上粘附、增殖,且两实验组与对照组在各时间点有显著性差异(P<0.01)。碱性磷酸酶活性各时间点在两实验组间无明显差异。结论:多孔支架材料PLGA/HA(5%HA)、PLGA/HA(10%HA)具有良好的生物相容性,有可能应用于骨组织工程。

Biocompatibility Studies on Porous PLGA/HA

Objective: To study the biocompatibility of porous PLGA/HA. Methods: Bone marrow stromal cells (BMSCs) were collected from rabbits, cultured ,induced and proliferated in vitro. BMSCs were cultured in extraction solution of PLGA/HA(with 5%HA and 10%HA)to evaluate the cytotoxicity; BMSCs were co-cultured with PLGA/HA(with 5%HA and 10%HA)and with PLGA as control group. Adhesion and proliferation on these scaffolds were examined qualitatively and quantitatively . Results: Cytotoxicity stage of porous materials was 0 or 1; BMSCs could be attached to and extended on the 3 scaffolds, and proliferated normally. Moreover, experimental groups were superior to control group in adhesive and proliferative abilities(P﹤0.01) Conclusion: The results show that porous PLGA/HA have good biocompatibility with BMSCs.