乳源免疫调节肽对酒精诱导性肝细胞损伤的干预机制

目的：研究乳源免疫调节肽( PGPIPN)对肝细胞酒精性脂肪变性的干预机制。方法 MTT法筛选正常肝细胞株( LO2)酒精诱导的最佳浓度和PGPIPN最佳用药范围,在肝细胞内加入酒精和不同浓度的PGPIPN进行干预,连续造模3个月,建立能稳定遗传的酒精诱导脂肪变性的肝细胞模型,观察PGPIPN对肝细胞脂肪变性的防治效果。油红O染色法观察LO2细胞脂肪变性程度,RT-PCR法检测脂肪合成相关基因( ACC、PPAR-γ)的表达。结果成功建立了能稳定遗传的酒精诱导脂肪变性的肝细胞模型,并筛选出酒精诱导LO2脂肪变性的最佳浓度和PGPIPN最佳用药范围。 PG-PIPN可以防治和缓解脂肪变性,PGPIPN可能通过降低ACC的基因表达,升高PPAR-γ的基因表达来实现。结论 PG-PIPN可防治和放缓肝细胞酒精性脂肪病变,机制可能是减少脂肪合成。

The effect and the mechanism of immunomodulating peptide on alcoholic fatty liver cells model

Objective To explore the effect of immunomodulating peptide ( PGPIPN ) on mechanism on alcoholic fatty liver cells model induced in vitro in human. Methods The liver cells ( LO2 ) proliferation with the optima con-centration of alcohol and PGPIPN in vitro were assayed by MTT method. Liver cells were treated with alcohol and different concentrations of PGPIPN for 3 months, then stable model of alcoholic fatty liver cells were established with screening concentration, aiming to observing the prevention and mitigation effects of PGPIPN on alcoholic fatty liver cell. Oil Red O staining was used to detect the degree of LO2 cells steatosis. The expressions of genes of fat synthesis related genes( ACC,PPAR-γ) were tested by RT-PCR. Results An alcohol induced fatty liver cells mod-el was established successfully. The best concentration of alcohol and PGPIPN was already screened. PGPIPN could prevent and mitigate alcoholic fatty degeneration of the liver cells. The mechanism may be associated with de-creasing expression of ACC ( mRNA) and increasing expression of PPAR-γ( mRNA) induced by PGPIPN. Conclu-sion PGPIPN can reduce alcoholic fatty degeneration of the liver cells, which may relate to slow fat synthesis.