骨肉瘤VEGF和PCNA表达与微血管密度的关系研究

目的　探讨血管新生在骨肉瘤生长、浸润、转移中的作用。方法　通过对 5 2例骨肉瘤组织标本的研究 ,采用抗人血管内皮生长因子VEGF (VascularEndothlialGrowthFactor)单克隆抗体、抗人增殖细胞核抗原PCNA(Pro1iferatingCellNuclearAntigen)单克隆抗体 ,借助免疫组织化学方法 ,对代表血管新生的肿瘤VEGF表达、微血管密度 (MicrovessleDensity)及代表肿瘤细胞增殖的PCNA的表达进行检测。 结果　VEGF表达和MVD与瘤转移密切相关 (P <0 .0 5 ) ,与肿瘤大小和肿瘤细胞分型无关 (P >0 .0 5 ) ;PCNA表达与骨肉瘤转移有关 (P <0 .0 5 )。结论　骨肉瘤体内血管新生不仅为肿瘤生长提供条件 ,也是其转移的基础。PCNA可作为术后肿瘤转移的预测因子及判断预后的指标

Expression of VEGF and PCNA and Microvessel Density in Osteosarcoma

Objective To study the role of the angiogenesis in osteosarcoma growth invasion and metastasis. Methods Immunohistochemical staining, using monoclonal antibodies was used to quantify the VEGF expression, the PCNA expression and the microvessel density(MVD)in 52 surgical specimens resected from patients with osteosarcoma. Anti-VEGF monochonal antibody was used to determine VEGF expression, anti-PCNA monoclonal antibody was used to determine PCNA expression. Results VEGF and MVD expression had significant correlation with metastasis ( P <0.05).VEGF and MVD expression had no correlation with tumor cell-type and tumor size ( P >0.05).PCNA expression had correlation with osteosarcoma metastasis ( P <0.05). Conclusion That angiogenesis is not only the basis of tumor of metastasis but also an important factor in tumor growth. PCNA expression could be the parameters in the judgment of postoperation metastasis and prognosis.