常氧培养与低氧培养肺间充质细胞凋亡的比较

背景：小鼠间充质细胞在体外培养扩增困难，细胞凋亡是否是其原因之一，目前未知。目的：观察常氧和低氧条件下，小鼠肺间充质细胞的凋亡率是否有差别。方法：用组织块培养法获得C57BL/6J雄性小鼠肺间充质细胞，分别在常氧(体积分数20%氧)和低氧(体积分数5%的氧)条件下培养，观察细胞内活性氧水平、凋亡率和超微结构的变化。结果与结论：体积分数为5%和20%氧气培养的细胞随代数的增加造血细胞均逐渐减少。体积分数20%氧气培养的小鼠肺间充质细胞内的活性氧水平明显高于体积分数5%氧气培养的细胞。体积分数20%氧气培养的肺间充质细胞的凋亡现象较常见，体积分数5%氧气培养的细胞凋亡现象较少见。提示常氧培养条件下，细胞的凋亡率增加，降低氧浓度可减少细胞的凋亡率。

Apoptosis of lung mesenchymal cells under normal oxygen culture versus low oxygen culture

BACKGROUND: Expansion of murine-derived mesenchymal cells is difficult in vitro and it is unclear whether this phenomenon is caused partly by cell apoptosis.OBJECTIVE: To compare the differences of mouse lung mesenchymal cell apoptosis between normal oxygen and low oxygen culture conditions. METHODS: C57BL/6J male mouse lung derived-mesenchymal cells were isolated by primary explant culture and cultured under normal (20% O2) and low oxygen (5% O2) conditions. The differences in intracellular active oxygen level, apoptotic rate and ultrastructure were observed.RESULTS AND CONCLUSION: The number of hematopoietic cells was decreased with increased cell passage under both 5% and 20% O2 condition. The active oxygen levels were significantly greater in the mouse lung-mesenchymal cells under 20% O2 condition compared with those under 5% O2 condition. Apoptosis was more in cells under 20% O2 condition compared with those under 5% O2 condition. These indicate that under normal oxygen culture condition, the apoptotic rate of cells increases and low oxygen can reduce the apoptosis.