广州地区猴腺病毒7型血清流行病学初步调查

目的评估广州地区猴腺病毒7型(SAdV-7)先存中和抗体阳性率情况。方法首先构建以β-半乳糖苷酶(LacZ)为报告基因,结合CMV启动子的猴重组腺病毒7型载体SAdV7-CMV-LacZ。然后使用化学发光法,检测209份免疫功能正常的成人的血清样本中SAdV-7中和抗体阳性率情况。结果我们发现SAdV-7中和抗体的阳性率为27.8%(58/209)。SAdV-7中和抗体在低滴度时阳性率最高,并且随着滴度的升高,阳性血清分布率逐渐降低。在20～40岁人群中,SAdV-7中和抗体阳性率最高,在<20岁人群中未检测到SAdV-7中和抗体。结论广州地区人群中针对SAdV-7的先存中和抗体阳性率低。因此,SAdV-7在基因治疗及基因疫苗的应用上是一个具有吸引力的候选载体。

Epidemiological survey of simian adenovirus type 7 in Guangzhou

Objective To evaluate the positive ratio of preexisting neutralizing antibodies of simian adenovirus type 7 (SAdV-7) in Guangzhou.Methods The simian recombinant adenovirus type 7 vector that was driven by CMV promoter and used β-galactosidase (LacZ) as reporter gene was constructed and the positive ratio of neutralizing antibodies of SAdV-7 in the serum of 209 immunocompetent individuals was evaluated using chemiluminescence method.Results Among the studied population,the ratio of individuals with SAdV-7 neutralizing antibodies was 27.8% (58/209).The SAdV-7 neutralizing antibodies were highly prevalent in low titer,the prevalence decreased as the titer increased.The positive ratio of SAdV-7 neutralizing antibodies were highest in the individuals who were at the age of 20 to 40.While SAdV-7 neutralizing antibodies in the individuals younger than 20 was not detected.Conclusion As the SAdV-7 neutralizing antibody is lowly prevalent in population of Guangzhou,it is a promising candidate vector for gene therapy and gene vaccine.