三氯异氰尿酸消毒片杀菌效果与毒性试验观察

为了解三氯异氰尿酸消毒片杀菌效果及毒性,以悬液定量杀菌试验和毒性试验进行了试验观察。结果,以含有效氯250 mg/L该消毒片溶液对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌作用15 m in,杀灭对数值均>5.0,对白色念球菌的杀灭对数值>4.0。含2500 mg/L有效氯该消毒液对枯草杆菌黑色变种芽孢作用30 m in,杀灭对数值>5.0。对铜绿假单胞菌能量试验最低合格浓度为500 mg/L。用含250 mg/L该消毒液浸泡作用30 m in,对瓷盘上大肠杆菌的杀灭对数值>3.0。以含1000 mg/L消毒液作用15 m in,对物体表面自然菌的杀菌对数值为1.72。该消毒片对小鼠经口毒性LD50为>5000 mg/kg,属实际无毒级;小鼠骨髓嗜多染红细胞微核试验阴性。结论,三氯异氰尿酸消毒片对细菌繁殖体和枯草杆菌黑色变种芽孢杀灭作用较强,动物试验属无毒级物质。

EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF TRICHLOROISOCYANURIC ACID DISINFECTION TABLET

In order to know the germicidal efficacy and toxicity of trichloroisocyanuric acid disinfection tablet,suspension quantitative germicidal test and toxicity test were used to carry out experimental observation.Results: The reduction factor(log_(10)) of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa exposed to the disinfection tablet solution containing available chlorine 250 mg/L for 15 min were >5.0 and the reduction factor(log_(10)) of Candida albicans was >4.0.The reduction factor(log_(10)) of spores of Bacillus subtilis var.niger exposed to the disinfection tablet solution containing available chlorine 2500 mg/L for 30 min was >5.0.The minimum eligible concentration for killing Pseudomonas aeruginosa in capacity test was 500 mg/L.The reduction factor(log_(10)) of E.coli on porcelain dish immersed in the disinfection tablet solution containing available chlorine 250 mg/L for 30 min was >3.0 and that of natural bacteria on surface of object exposed to the disinfection tablet solution containing available chlorine 1000 mg/L for 15 min was 1.72.The mouse oral LD_(50) of this disinfection tablet was >5000 mg/kg,indicating that it is practically non-toxic.It was negative in mouse bone marrow polychromatic erythrocyte micronucleus test.Conclusions: Trichloroisocyanuric acid disinfection tablet has relatively strong killing effect on vegetative forms of bacteria and spoces of Bacillus subtilis var.niger.Animal test showed that it belongs to non-toxic substances.