| RNA干扰及其技术的应用 |
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| 引用本文： | 钱颖,范春雷.RNA干扰及其技术的应用[J].浙江中医药大学学报,2006,30(1):104-106. |
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| 作者姓名： | 钱颖 范春雷 |
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| 作者单位： | 浙江中医学院,杭州,10053 |
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| 摘 要： | RNA干扰(RNAi)是细胞内由双链RNA诱导降解与其配对的特定mRNA的过程。细胞内双链RNA在酶的作用下,形成20-25碱基大小的小干扰RNA(si RNAs),由si RNAs进一步掺入多组分核酸酶并使其激活,从而精确降解与si RNAs序列相同的mRNA,抑制该基因在细胞内的翻译表达。介绍了RNA干扰的分子机制、制备方法、以及RNA干扰技术在功能基因组学、微生物学、基因治疗和信号转导等研究领域里的应用。
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| 关 键 词： | RNAi 转录后基因沉默 基因沉默复合体 基因功能 信号转导 基因治疗 |
| 文章编号： | 1005-5509（2006）01-0104-03 |
| 收稿时间： | 2005-03-30 |
| 修稿时间： | 2005-03-30 |
RNAi and Its Application |
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| Qian Ying,Fan Chunlei.RNAi and Its Application[J].Journal of Zhejiang University of Traditional Chinese Medicine,2006,30(1):104-106. |
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| Authors: | Qian Ying Fan Chunlei |
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| Abstract: | RNA interfere(RNAi)is a process in which double-stranded RNA triggers the degradation of a homologous mRNA.The double-stranded RNA in cell is diced-up a class of 20-25 nucleotide-long RNA(siRNA)by the enzyme Dicer.The siRNA degrades its homologous mRNA to interfere with the gene expression by mixing with and activating multi-component nucleases.Here it introduces the mechanism of RNAi,its methods of preparation and its application on research fields,such as functional genomics,microbiology,gene therapy and signal transduction,etc. |
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