Measurement of the platelet retention rate in a column of collagen-coated beads is useful for the assessment of efficacy of antiplatelet therapy |
| |
Authors: | Akira Mende Jyun-ei Obata Keita Sano Mitsumasa Hirano Yoshinobu Kitta Yasushi Kodama Takamitsu Nakamura Ken-ichi Kawabata Yukio Saitoh Daisuke Fujioka Tsuyoshi Kobayashi Kaneo Satoh Yukio Ozaki Toshiaki Yano Kiyotaka Kugiyama |
| |
Institution: | aDepartment of Internal Medicine II, University of Yamanashi, Chuo city, Yamanashi prefecture, Japan;bDepartment of Clinical and Laboratory Medicine, University of Yamanashi, Chuo city, Yamanashi prefecture, Japan |
| |
Abstract: | IntroductionA simple, validated method to measure platelet function is unavailable for bedside use. Measurement of platelet retention rate using a column of collagen-coated beads and whole blood is a new, simple assay that reflects platelet aggregation. This study was aimed to examine the utility of this assay to assess efficacy of antiplatelet drug therapy.MethodsCitrated whole blood (1.5 ml) in a syringe was passed through a polyvinyl tube packed with collagen-coated beads for 40 seconds using a syringe pump. Platelet retention rate in the column was calculated from platelet counts in blood before and after passage. An increase in the retention rate reflects an increase in platelet activity. This new platelet retention assay and the traditional optical aggregometry assay were performed in 331 patients with stable coronary artery disease (CAD).ResultsThe retention rate was significantly reduced in patients taking dual antiplatelet therapy (aspirin plus clopidogrel or ticlopidine) compared with aspirin alone. There was a significant linear correlation between the platelet retention rate and platelet aggregability measured by the traditional method (r = 0.44, p < 0.001). In multivariate Cox proportional hazards analysis, higher platelet retention rate was an independent predictor of future cardiovascular events in patients on dual antiplatelet therapy (hazard ratio 3.9, 95% CI 1.6 to 9.5, p = 0.003).ConclusionsMeasurement of the platelet retention rate in a column of collagen-coated beads may be useful for monitoring the efficacy of antiplatelet drug therapy in patients with CAD. |
| |
Keywords: | Platelet function Antiplatelet drugs Coronary artery disease |
本文献已被 ScienceDirect 等数据库收录! |
|