AP-PCR法对沙门菌第一类整合子鉴定指纹图谱分析

目的在研究沙门菌携带的第一类整合子基因结构的基础上，对第一类整合子的阳性菌株进行基因指纹图谱分析。方法任意引物聚合酶链反应（AP-PCR）方法对两种不同血清型、整合子阴性与整合子阳性菌株研究基因指纹图谱，利用遗传距离矩阵，采用非加权配对法（UPGMA法）做遗传分析的树状图，进行指纹图谱分析。结果在最佳AP-PCR方法的反应条件下，可以将第一类整合子阴性、第一类整合子阳性菌株进行鉴定，同时对两种不同的血清型也可以加以区分，得到5个基因型。结论AP-PCR可以作为第一类整合子阳性菌株及其相关血清型进行尝试性的鉴定方法，对研究整合子介导细菌耐药机制的特性具有重要意义。

Fingerprinting genomes of class 1 integron among Salmonella strains using PCR with arbitrary primers

Objective The role of integrons and gene cassettes in the spread of antibiotic resistance is well established. The characterization of class 1 integron among Salmonella stains had been well investigated previously. Fingerprinting genomes of those Salmonella strains were studied using PCR with arbitrary primers(AP- PCR). Method Two kinds of serovar strains, integron- positive isolates and integron- negative isolates with different characterization were used as the samples. The fingerprinting of those strains were investigated by the method of AP- PCR. Bendrogram of unweighted paired- growp method using arithmetic average (UPGMA) cluster analysis based on polymorphism data was obtained by UPGMA analysis. Result Five gene types of the strains were classified by the UPGMA analysisaccording to their fingerprint. Conclusion The AP- PCR fingerprinting was a good method to identify different characterizations of integrons and serovar strains for research of mechanisms of antibiotic resistance in bacteria.