Expression of p53, VEGF, Microvessel Density, and Cyclin-D1 in Noncancerous Tissue of Inflammatory Bowel Disease |
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Authors: | Canan Alkim Berna Savas Arzu Ensari Huseyin Alkim Ulku Dagli Erkan Parlak Aysel Ulker and Burhan Sahin |
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Institution: | (1) Gastroenterology Department, Sisli Etfal Research and Training Hospital, Istanbul, Turkey;(2) Nisantasi Ihlamur Yolu No: 51-1, Unsal Karahan Sitesi, C Blok, D: 49, Tesvikiye, Sisli, 34365 Istanbul, Turkey;(3) Faculty of Medicine, Department of Pathology, Ankara University, Ankara, Turkey;(4) Gastroenterology Department, Alman Hospital, Istanbul, Turkey;(5) Gastroenterology Department, Turkey High Speciality Hospital, Ankara, Turkey |
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Abstract: | We aimed to evaluate the carcinogenesis risk in inflammatory bowel disease via p53 mutation and its relation with hyperproliferation
(cyclin-D1) and angiogenesis (with vascular endothelial growth factor VEGF] and microvessel density) and whether these events
play important roles in pathogenesis of inflammatory bowel disease. Colonic tissue samples of 26 ulcerative colitis, 6 Crohn’s
disease, and 8 amoebic colitis patients as well as samples of 10 healthy controls were stained with p53, cyclin-D1, CD34,
and VEGF monoclonal antibodies by immunohistochemistry and evaluated semiquantitatively. Expression of p53 was higher in ulcerative
colitis than in the healthy control and amoebic colitis groups (4.15 ± 2.07, 1.4 ± 1.5, 1.3 ± 1.5; P < 0.001). The Crohn’s disease group had the highest p53 expression (4.6 ± 1.6). The Crohn’s disease, ulcerative colitis,
and amoebic colitis groups all had higher VEGF expression than did the healthy controls (respectively, 4.3 ± 1.2, 2.92 ± 2.0,
2.3 ± 1.5, 0.6 ± 0.97; P < 0.001). Also, microvessel density was statistically higher in all three colitis groups than in healthy controls. Cyclin-D1
expression in all four groups was similar. The study showed that p53 mutation was present in nonneoplastic mucosa of inflammatory
bowel disease patients. Detecting strong p53 overexpression with VEGF overexpression may help in differentiating inflammatory
bowel disease from other colitis. |
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Keywords: | Carcinogenesis Inflammatory bowel disease p53 VEGF and microvessel density Cyclin-D1 |
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