| Abstract: | AIM:To investigate the effects of vitamins(A,C and F)on liver injury induced by ethanol administration during liver regeneration in rats.METHODS:Male Wistar rats subjected to 70% partial hepatectomy were divided into five groups (groups 1-5).During the experiment,animals of Group 1 drank only water.The other four groups(2-5)drank 30 mL of ethanol/L of water.Group 3 additionally received vitamin A,those of group 4 vitamin C and those of group 5 received vitamin E.Subsequently serum alanine aminotTansferase (ALT),aspartate aminotransferase (AST),albumin and bilirubin were measured colorimetrically.Lipid peroxidation (thiobarbituric-acid reactive substances,TBARS) both in plasma and liver was measured,as well as liver mass gain assessment and total DNA.RESULTS;Compared with sham group,serum AST and ALT increased significantly under ethanol treatment (43% and 93%,respectively,with P＜0.05).Vitamin C and vitamin E treatment attenuated the ethanol-induced increases in ALT and AST activity.Ethanol treatment also decreased serum albumin concentration compared to sham group (3.1 ± 0.4 g/dL vs 4.5 ± 0.2 g/dL;P ＜ 0.05).During liver regeneration vitamins C and E significantly ameliorated liver injury for ethanol administration in hepatic lipid peroxidation (4.92 nmol/mg and 4.25 nmol/mg vs 14.78 nmol/mg,respectively,with P ＜ 0.05).In association with hepatic injury,ethanol administration caused a significant increase in both hepatic and plasma lipid peroxidation.Vitamins (C and E) treatment attenuated hepatic and plasma lipid peroxidation.CONCLUSION:Vitamins C and E protect against liver injury and dysfunction,attenuate lipid peroxidation,and thus appear to be significantly more effective than vitamin A against ethanol-mediated toxic effects during liver regeneration. |
