A new assay for interleukin-1 in the presence of interleukin-2 |
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| Authors: | W Falk P H Krammer D N M?nnel |
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| Affiliation: | 1. Association of Dutch Burn Centres, P.O. Box 1015, 1940 EA Beverwijk, The Netherlands;2. Department of Psychology, Utrecht University, P.O. Box 80140, 3508 TC Utrecht, The Netherlands;3. Burn Centre Red Cross Hospital, Vondellaan 13, 1942 LE Beverwijk, The Netherlands;4. Burn Centre Maasstad Hospital, Maasstadweg 21, 3079 DZ Rotterdam, The Netherlands;1. UR05/08-08, Department of Biochemistry, Rabta Hospital, Tunis, Tunisia;2. Service of Neonatology, Center of Maternity and Neonatology, Faculty of Medicine of Tunis, El Manar University, Tunis, Tunisia |
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| Abstract: | A simple and reliable assay for interleukin-1 (IL-1) is described which has the advantage over other assays that it is independent of interleukin-2 (IL-2) production by the test cells. The assay makes possible the detection of IL-1 in the supernatants of T cell populations. The ability of IL-1 to induce IL-2 receptor expression in the absence of T cell mitogen is the basis of this assay. Thus, proliferation of mouse thymocytes incubated in the presence of saturating concentrations of IL-2 (10 U/ml) was directly dependent on the concentration of IL-1. The sensitivity of the assay is comparable to the sensitivity of the classical thymocyte co-stimulator assay. Natural and recombinant human and murine IL-1 were measured in this test system with comparable sensitivity. |
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