肺纤方提取物对肺间质纤维化大鼠血管新生的影响

目的探讨肺纤方提取物对肺间质纤维化大鼠肺纤维化程度和肺微血管变化和血小板-内皮黏附因子31、34(CD31、CD34)表达的影响。方法将大鼠分为假手术组、模型组、泼尼松组、氯沙坦组和肺纤方大、中、小剂量组,共7组。除假手术组外,采用气管插管灌注博莱霉素3 mg/kg进行大鼠肺间质纤维化造模;造模第2天予以不同药物灌胃干预。于14、28 d分两批进行动物处理,电镜观察肺纤维化程度和肺微血管变化,免疫组化检测CD31、CD34表达。结果肺纤方大、中剂量组均能抑制肺泡炎、纤维化程度及微血管密度,较模型组有显著差异(P0.05)。结论肺纤方通过减轻肺纤维化微血管密度表达,减少肺泡炎时的异常血管新生,而具有抗肺间质纤维化作用。

Effect of Feixian Fang extract on angiogenesis of pulmonary interstitial fi-brosis rats

Objective Feixian Fang is a Chinese medicine compound,formulated by the principle of in-vigorating lung and kidney,reducing phlegm and resolving masses,and promoting blood circulation to re-move meridian obstruction.This paper aimed to investigate the effect of Feixian Fang extract (FXF)on degree of pulmonary fibrosis,change of microvessels and platelet endothelial cell adhesion molecules (CD 31,CD34)in rats with pulmonary interstitial fibrosis.Methods All rats were divided into sham opera-tion group,model group,prednisone group,losartan group,FXF high-,mid-and low-dose group.Ex-cept the sham operation group,the rats of other six groups were instilled bleomycin of 3 mg/kg through tracheal intubation.The next day,the rats were administered intragastrically corresponding medicines for consecutive 14 d or 28 d.The degree of pulmonary fibrosis,change of microvessels were observed under electron microscope and the expression of CD31 and CD34 were detected using immuhistochemistrical methods.Results High dose and middle dose FXF inhibited the pulmonary fibrosis and pulmonary alve-olar inflammation and decreased microvascular density (P <0.05).Conclusion The effect of FXF a-gainst pulmonary interstitial fibrosis can be via the way of decreasing microvascular density to induce less abnormal angiogenesis in pulmonary fibrosis.