大鼠脊髓再灌注损伤中三甲氧苄嗪的保护作用

目的　评价三甲氧苄嗪对大鼠脊髓再灌注损伤的保护作用 ,并探讨其作用机制。方法　 4 5只SD大鼠随机分为 3组 ,假手术组、对照组和三甲氧苄嗪组 ,每组 15只。建立大鼠脊髓缺血损伤模型 ,假手术组不阻断主动脉 ,对照组在肾动脉以下及髂动脉以上同时阻断主动脉 2 0min ,三甲氧苄嗪 (TMZ)组于主动脉阻断前 10min静脉内注射三甲氧苄嗪 (3mg/kg) ,余同对照组 ,2 0min后开放主动脉。分别于阻断主动脉前、松开主动脉前和松开主动脉后2h取静脉血测定丙二醛 (MDA)含量。术后 4 8h按Tarlov评分标准评价动物后肢神经功能 ,然后处死动物 ,取脊髓进行含水量、MDA含量测定及组织病理学检查。结果　TMZ组血中MDA含量明显低于对照组 (P <0 .0 5 ) ;TMZ组动物后肢神经功能评分明显高于对照组 (P <0 .0 1) ;TMZ组脊髓含水量、MDA含量明显低于对照组 (P <0 .0 1) ;TMZ组光镜下脊髓病理改变轻微 ,而对照组较重 ,两组病理评分有显著性差异 (P <0 .0 1)。结论　三甲氧苄嗪对大鼠脊髓缺血再灌注损伤具有明显的保护作用

The Protective Effect of Trimetazidine on Reperfusion Injury of Spinal Cord in Rats

OBJECTIVE To evaluate the effect of trimetazidine on reperfusion injury of spinal cord in rats and discuss its mechanism .METHODS With rat spinal cord ischemia model , we induced ischemia by clamping the aorta just distal to the renal artery and above the iliac bifurcation for 20 min . Forty-five spraque-dawley rats were randomly assigned to three groups(n=15 in each group ) . Sham-operation group subjected to operation dissections without aortic occlusion ; control group undergoing occlusion but receiving no treatment ; TMZ (trimetazidine)group receiving 3mg/kg trimetazidine intravenously in ten minutes before the occlusion of aorta. The malondialdehyde(MDA) content in blood was measured before the occlusion, during the ischemia and after 2 hours of reperfusion respectively. Neurologic status was assessed in 48h after operation , then all animal were sacrificed and the water content , MDA content of spinal cord were also measured . The spinal cord was processed for histopathologic examination . RESULTS Compared with the control group , the TMZ group had lower water content and MDA content (P<0.01) . The neurological scoring of TMZ group was significantly higher than that of control group (P<0.01) . The histopathologic change of spinal cord in TMZ group was milder compared with that in control group (P<0.01) . CONCLUSION Trimetazidine could protect the rat spinal cord from ischemic-reperfusion injury .