| 环己米特不能阻止α—双炔失碳酯诱导的白血病K562细胞凋亡 |
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| 引用本文： | 楼丽广,胥彬.环己米特不能阻止α—双炔失碳酯诱导的白血病K562细胞凋亡[J].中国药理学报,1997,18(2):169-172. |
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| 作者姓名： | 楼丽广 胥彬 |
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| 作者单位： | 中国科学院上海药物研究所 |
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| 摘 要： |
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| 关 键 词： | α－双炔失碳酯 环己米特 白血病 K562 DNA损伤 |
alpha-Anordrin-induced apoptosis of leukemia K562 cells is not prevented by cicloheximide. |
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| L G Lou,B Xu.alpha-Anordrin-induced apoptosis of leukemia K562 cells is not prevented by cicloheximide.[J].Acta Pharmacologica Sinica,1997,18(2):169-172. |
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| Authors: | L G Lou B Xu |
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| Institution: | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China. |
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| Abstract: | AIM: To study effect of protein synthesis inhibitor cicloheximide (Cic) on the apoptosis induced by alpha-anordrin (Ano) in leukemia K562 cells. METHODS: Morphological changes were observed by fluorescent microscopy. DNA content was measured by flow cytometry. DNA fragmentation was analyzed by agarose gel electrophoresis. RESULTS: Exposure of K562 cells to Ano 50 mumol.L-1 for 24 h induced apoptotic cell death. Cic 1 mumol.L-1 did not abrogate or delay this effect. Indeed, Ano-induced apoptosis was augmented by Cic. Cic 100 mumol.L-1 itself stimulated 25% K562 cell apoptosis after 24-h culture. CONCLUSION: Ano-induced apoptosis was independent of de novo protein synthesis. |
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