| β—胡萝卜素对^60Co—γ辐射诱发的大鼠T—淋巴细胞次黄嘌呤—鸟嘌呤… |
| |
| 引用本文： | 倪庆桂 裴云. β—胡萝卜素对^60Co—γ辐射诱发的大鼠T—淋巴细胞次黄嘌呤—鸟嘌呤…[J]. 中国药理学报, 1997, 18(6): 535-536 |
| |
| 作者姓名： | 倪庆桂 裴云 |
| |
| 作者单位： | 海军神经生物学研究中心分子药理室 |
| |
| 摘 要： | 目的：研究β－胡萝卜素（β－Ｃａｒ）对电离辐射诱导的突变的影响。方法：Ｔ－淋巴细胞克隆检测法测定大鼠Ｔ－淋巴细胞次黄嘌呤－鸟嘌呤磷酸核糖转移酶（ＨＧＰＲＴ）位点的突变率和硫代巴比妥酸（ＴＢＡ）法测定大鼠血丙二醛（ＭＤＡ），结果：＾６０Ｃｏγ３．７５Ｇｙ照射大鼠显著提高ＨＧＰＲ位点突变频率，ｉｇβ－Ｃａｒ１０和２０ｍｇ．ｋｇ＾－１突变频率明显降低（Ｐ〈０．０５），应用β－Ｃａｒ５ｍｇ．ｋｇ＾－１变化
|
| 关 键 词： | 胡萝卜素 辐射损伤 次黄嘌呤 磷酸核糖转移酶 |
Effect of beta-carotene on 60Co-gamma-induced mutation at T-lymphocyte hypoxanthine-guanine phosphoribosyl transferase locus in rats] |
| |
| Q G Ni,Y Pei. Effect of beta-carotene on 60Co-gamma-induced mutation at T-lymphocyte hypoxanthine-guanine phosphoribosyl transferase locus in rats][J]. Acta Pharmacologica Sinica, 1997, 18(6): 535-536 |
| |
| Authors: | Q G Ni Y Pei |
| |
| Affiliation: | Department of Molecular Pharmacology, Naval Neurobiology Research Centre, Nanjing, China. s5922599@public.ptt.js.cn |
| |
| Abstract: | AIM: To study the effect of beta-carotene (beta-Car) on radiation-induced mutation. METHODS: Mutant frequency at T-lymphocyte hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in rats was measured using a T-lymphocyte cloning technique and malondialdehyde (MDA) was measured using a thiobarbituric acid (TBA). RESULTS: Mutant frequency at the HGPRT locus was elevated by 60Co-gamma 3.75 Gy, and was reduced by ig beta-car 20 and 10 mg.kg-1 (P < 0.05), but not by beta-Car 5 mg.kg-1. It showed a dose-dependent relationship. MDA was signifcantly reduced in plasma of rats given beta-Car after 60 Co-gamma radiation. There was positive coherence between MDA and mutant frequency at the HGPRT locus (r = 0.9978, P < 0.05). CONCLUSIONS: Radiation-induced mutation is inhibited by beta-Car, associated with antioxidant action of beta-Car. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
