| 粉防己碱对大鼠大脑c—fos原癌基因表达的影响 |
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| 引用本文： | 车建途,张均田.粉防己碱对大鼠大脑c—fos原癌基因表达的影响[J].中国药理学报,1997,18(4):371-373. |
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| 作者姓名： | 车建途 张均田 |
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| 作者单位： | 中国医学科学院 |
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| 摘 要： | 目的：本研究旨在探讨Ｔｅｔ对林丹－一种通过细胞内游离钙（Ｃａ＾２＋）ｉ）介导大脑ｃ－ｆｏｓ基因表达的神经毒剂－诱导大鼠大脑ｃ－ｆｏｓ基因表达的影响。方法：Ｎｏｒｔｈｅｒｎ印迹杂交斑点杂交技术及双波长薄层长色谱扫功分析，结果：大鼠口服林丹３０ｍｇ．ｋｇ＾－１小时后大脑ｃ－ｆｏｓ基因表达明显增强至１４６ｍｍ＾２而在口服林丹前３０ｍｉｎ分别口服Ｔｅｔ１，２及４ｍｇ．ｋｇ＾－１大鼠大脑ｃ－ｆｏｓ基因表达被
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| 关 键 词： | 粉防己碱 林丹 原癌基因蛋白 基因表达 |
Effect of tetrandrine on proto-oncogene c-fos expression in rat cerebrum. |
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| J T Che,J T Zhang,Z W Qu,G Wei.Effect of tetrandrine on proto-oncogene c-fos expression in rat cerebrum.[J].Acta Pharmacologica Sinica,1997,18(4):371-373. |
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| Authors: | J T Che J T Zhang Z W Qu G Wei |
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| Institution: | Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing, China. |
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| Abstract: | AIM: To detect the effect of tetrandrine (Tet) on c-fos gene expression in cerebrum induced by lindane, a neurotoxicant which activates Ca2+ channels. METHODS: Northern and dot blotting, dual wavelength thin layer chromatography scanner, were used in this study. RESULTS: Lindane 30 mg.kg-1 given by intragastric gavage (i.g.) increased the expression of c-fos gene to 146 mm2 in rat cerebrum 1 h after treatment. Tet 1, 2, and 4 mg.kg-1 given by i.g. 30 min prior to lindane reduced c-fos gene expression in a concentration-dependent manner. Expressed genes reached only 86, 40, and 39 mm2, respectively. CONCLUSION: Tet inhibited c-fos gene expression in rat cerebrum induced by Ca2+ agonist-lindane. |
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