降钙素基因相关肽预适应对溶血磷脂酰胆碱抑制内皮依赖性舒张的…

目的：研究降钙素基因相关肽（ＣＧＲＰ）诱导预适应对溶血磷脂酰胆碱（Ｌｙｓ）抑制内皮依赖性舒张的作用。方法：用苯福林收缩兔与大鼠离体胸主动脉环，观察Ｌｙｓ对乙酰胆碱（ＡＣｈ）所致内皮依赖性舒张的影响。结果：ＣＧＲＰ预处理兔和大鼠离体胸主动脉环显著减轻Ｌｙｓ对ＡＣｈ舒血管效应的抑制，其作用可被蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｈ－７所取消。结论：ＣＧＲＰ诱导预适应对所致内皮细胞损伤具有拮抗作用，此作用与激活Ｐ

Effect of calcitonin gene-related peptide-induced preconditioning on attenuated endothelium-dependent vasorelaxation induced by lysophosphatidylcholine.

AIM: To study the effects of calcitonin gene-related peptide (CGRP)-induced preconditioning on the inhibition of endothelium-dependent vasorelaxation to acetylcholine (ACh) by lysophosphatidylcholine (Lys) in the isolated rabbit and rat thoracic aortas. METHODS: Endothelium-dependent relaxation to ACh was studied in the aortic rings precontracted with phenylephrine 0.1 mumol.L-1 in the absence or presence of Lys. RESULTS: On the rabbit aortic rings, Lys 5 mg.L-1 impaired vasodilator responses to ACh. Pretreatment with CGRP 0.1 mumol.L-1 for 5 min attenuated the inhibition of vasodilator responses to ACh by Lys. The effect of CGRP was blocked by 1-(5-isoquinolinysulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C (PKC) (% relaxations to ACh 1 mumol.L-1 were 88 +/- 4, 28 +/- 10, 65 +/- 13, and 25 +/- 10 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively). The same effects of CGRP were shown in the rat aortic rings, and the effect of CGRP was also abolished by H-7 (% relaxations to ACh 1 mumol.L-1 was 84 +/- 10, 55 +/- 11, 76 +/- 11, and 50 +/- 14 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively). CONCLUSION: CGRP-induced preconditioning protected the endothelium against injury elicited by Lys, the effect of CGRP is related to the activation of PKC.