| 16S rRNA在检测肺炎克雷伯菌引起血流感染中的应用 |
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| 引用本文： | 张志明,李建平,朱建伟.16S rRNA在检测肺炎克雷伯菌引起血流感染中的应用[J].中国热带医学,2011,11(3):272-273. |
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| 作者姓名： | 张志明 李建平 朱建伟 |
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| 作者单位： | 西安市中心医院,检验科,陕西,西安,710003 |
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| 基金项目： | 陕西省卫生厅科学研究基金项目 |
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| 摘 要： | 目的初步建立一种对肺炎克雷伯菌引起血流感染的快速检测方法。方法应用实时荧光定量PCR（Real-time PCR）,从血培养阳性瓶中提取细菌基因组DNA,以特异性引物扩增16S rRNA靶片段,同期进行细菌培养鉴定的对照。结果检测142份已知肺炎克雷伯阳性标本,65份非肺炎克雷伯阴性对照,52份双盲样本显示敏感性为100%（146/146）特异性为100%（113/113）,整个实验可在2h内完成。结论较传统的培养鉴定法而言,以16SrRNA作为实时荧光定量靶基因可快速检测肺炎克雷伯菌,具有很好的研究价值与应用前景。
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| 关 键 词： | 16SrRNA 肺炎克雷伯菌 血流感染 荧光定量PCR |
Application of 16S rRNA in the detection of bloodstream infections with Klebsiella pneumoniae. |
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| ZHANG Zhi-ming,LI Jian-ping,ZHU Jian-wei.Application of 16S rRNA in the detection of bloodstream infections with Klebsiella pneumoniae.[J].China Tropical Medicine,2011,11(3):272-273. |
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| Authors: | ZHANG Zhi-ming LI Jian-ping ZHU Jian-wei |
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| Institution: | ZHANG Zhi- ming,LI Jian-ping,ZHU Jian-wei.(Laboratory Department,Xi'an Municipal Central Hospital,Xi'an 710003,Shanxi,P.R. China) |
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| Abstract: | Aim To establish a methods for rapid detection of bloodstream infections with Klebsiella pneumoniae. Methods Real-time fluorescence quantitative PCR(Real-time PCR)was used for detection of bacterial genomic DNA with amplified 16S rRNA target fragments in samples extracted from the blood culture positive bottles and compared to bacterial culture and identification of the control.Results The sensitivity and specificity of detection of 142 samples positive for Klebsiella pneumoniae,65 control samples negative ... |
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| Keywords: | 16S rRNA |
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