| Abstract: | Since bacteria remain in the dentin following caries removal, restorative materials
with antibacterial properties are desirable to help maintaining the residual
microorganisms inactive. The adhesive system Clearfil Protect Bond (PB) contains the
antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) in its
primer, which has shown antimicrobial activity. However, its bactericidal effect
against biofilm on the dentin has been little investigated.ObjectiveThe aim of this study was to analyze by confocal laser scanning microscopy (CLSM)
and viable bacteria counting (CFU) the MDPB bactericidal effect against S.
mutans biofilm on the dentin surface. Material and MethodsBovine dentin surfaces were obtained and subjected to S. mutans
biofilm formation in BHI broth supplemented with 1% (w/v) sucrose for 18 h.
Samples were divided into three groups, according to the primer application (n=3):
Clearfil Protect Bond (PB), Clearfil SE Bond, which does not contain MDPB, (SE)
and saline (control group). After the biofilm formation, Live/Dead stain was
applied directly to the surface of each sample. Next, 10 µL of each primer were
applied on the samples during 590 s for the real-time CLSM analysis. The
experiment was conducted in triplicate. The primers and saline were also applied
on the other dentin samples during 20, 90, 300 and 590 s (n=9 for each group and
period evaluated) and the CFU were assessed by colonies counting. ResultsThe results of the CLSM showed that with the SE application, although non-viable
bacteria were detected at 20 s, there was no increase in their count during 590 s.
In contrast, after the PB application there was a gradual increase of non-viable
bacteria over 590 s. ConclusionsThe quantitative analysis demonstrated a significant decrease of S.
mutans CFU at 90 s PB exposure and only after 300 s of SE application.
Protect Bond showed an earlier antibacterial effect than SE Bond. |
