粗叶悬钩子总生物碱对急性肝损伤大鼠肝组织CYP2E1与CYP3A1酶mRNA表达的影响

目的 研究粗叶悬钩子总生物碱对肝药物代谢酶CYP2E1和CYP3A1 mRNA表达的影响。

Effect of Total Alkaloids of Rubus alceaefolius Poiron on Gene Expressions of CYP2E1 and CYP3A1 in Rats with Acute Liver Injury

Objective To explore the effects of total alkaloids of Rubus alceaefolius Poiron （RAP） on gene expressions of drug-metabolic enzymes, CYP2E1 and CYP3A1 in liver. Methods Sixty SD rats were randomly divided into six groups （ 10 rats in each）, the blank control group, the model control group, the biphendate group and the three RAP treated groups treated respectively with low-, middle- and high-dose of RAP. The model of acute hepatic injury was established with intra-peritoneal injection of carbon tetrachloride. Serum levels of ala- nine aminotransferase （ALT） and aspartate aminotransferase （AST）, and severity of hepatic tissue injury were measured, and the mRNA expressions of CYP2E1 and CYP3A1 in liver tissue were detected by RT-PCR. Results As compared with the model group, serum levels of ALT and AST were significantly lower in the high- and middle-dose ARP group （ P 〈0. 01 ）, but in the low-dose group, only ALT was significantly lower （ P 〈0. 01 ） ; the Severity of liver injury was milder in the RAP groups （ P 〈0.01 ） ; and both CYP2E1 and CYP3A1 mRNA expressions in liver were significantly lower in the biphendate and all RAP treated groups （ P〈0.01 or P〈0.05）. Conclusion RAP could significantly reduce the ALT and AST levels, protect liver cells from injury, and inhibit the mRNA expressions of CYP2E1 and CYP3A1 in liver tissue.