粗叶悬钩子总生物碱对急性肝损伤大鼠肝组织CYP2E1与CYP3A1酶mRNA表达的影响 |
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引用本文: | 洪振丰,李天骄,赵锦燕,林久茂,周建衡,胡娟.粗叶悬钩子总生物碱对急性肝损伤大鼠肝组织CYP2E1与CYP3A1酶mRNA表达的影响[J].中国中西医结合杂志,2009,29(8):711-715. |
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作者姓名: | 洪振丰 李天骄 赵锦燕 林久茂 周建衡 胡娟 |
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作者单位: | 1. 福建中医学院,福州,350108 2. 福建中西医结合研究院 |
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基金项目: | 福建省自然科学基金,陈可冀中西医结合发展基金,福建省高校中西医结合基础重点实验室开放课题基金 |
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摘 要: | 目的 研究粗叶悬钩子总生物碱对肝药物代谢酶CYP2E1和CYP3A1 mRNA表达的影响。
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关 键 词: | 粗叶悬钩子 总生物碱 CYP2E1 CYP3A1 肝损伤 |
Effect of Total Alkaloids of Rubus alceaefolius Poiron on Gene Expressions of CYP2E1 and CYP3A1 in Rats with Acute Liver Injury |
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Authors: | HONG Zhen-feng LI Tian-jiao ZHAO Jin-yan |
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Institution: | HONG Zhen-feng, LI Tian-jiao, ZHAO Jin-yan, et al( Fujian College of Traditional Chinese Medicine, Fuzhou 350108) |
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Abstract: | Objective To explore the effects of total alkaloids of Rubus alceaefolius Poiron (RAP) on gene expressions of drug-metabolic enzymes, CYP2E1 and CYP3A1 in liver. Methods Sixty SD rats were randomly divided into six groups ( 10 rats in each), the blank control group, the model control group, the biphendate group and the three RAP treated groups treated respectively with low-, middle- and high-dose of RAP. The model of acute hepatic injury was established with intra-peritoneal injection of carbon tetrachloride. Serum levels of ala- nine aminotransferase (ALT) and aspartate aminotransferase (AST), and severity of hepatic tissue injury were measured, and the mRNA expressions of CYP2E1 and CYP3A1 in liver tissue were detected by RT-PCR. Results As compared with the model group, serum levels of ALT and AST were significantly lower in the high- and middle-dose ARP group ( P 〈0. 01 ), but in the low-dose group, only ALT was significantly lower ( P 〈0. 01 ) ; the Severity of liver injury was milder in the RAP groups ( P 〈0.01 ) ; and both CYP2E1 and CYP3A1 mRNA expressions in liver were significantly lower in the biphendate and all RAP treated groups ( P〈0.01 or P〈0.05). Conclusion RAP could significantly reduce the ALT and AST levels, protect liver cells from injury, and inhibit the mRNA expressions of CYP2E1 and CYP3A1 in liver tissue. |
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Keywords: | CYP2E1 CYP3A1 |
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