甘草酸对人乳腺癌细胞增殖抑制和诱导凋亡作用的研究

目的　探讨甘草酸对人乳腺癌细胞 (MCF 7)增殖抑制和诱导凋亡的作用 ,并探讨凋亡发生与细胞内Ca2 浓度( [Ca2 ]i)变化的关系。方法　 2 .5～ 12 .5mmol/L甘草酸处理MCF 7细胞 2 4h ,采用MTT比色法测定细胞增殖能力。 5 .0mmol/L、7.5mmol/L和 10 .0mmol/L甘草酸处理MCF 7细胞 2 4h ,用末端脱氧核苷转移酶介导dUTP末端标记法和AnnexinV流式细胞仪法检测凋亡细胞。 7.5mmol/L甘草酸处理MCF 7细胞 2 4h ,采用Fura 2荧光负载方法测定 [Ca2 ]i的变化。结果　甘草酸从 5 .0mmol/L浓度起对MCF 7细胞的增殖抑制率显著升高 (P <0 .0 1) ,呈剂量依赖性 ;半增殖抑制浓度 (IC50 )为 15 .8mmol/L。 7.5mmol/L和 10 .0mmol/L甘草酸使细胞凋亡率显著升高 (P <0 .0 5和P <0 .0 1)。甘草酸处理组的 [Ca2 ]i明显低于对照组 (P <0 .0 5 )。结论　甘草酸具有抑制MCF 7细胞增殖和诱导凋亡的作用 ;其诱导细胞凋亡可能与细胞内Ca2 水平下调有关

Proliferative Inhibition and Apoptotic Induction Effect of Glycyrrhizin on Human Breast Carcinoma Cells

Objective To investigate the proliferative inhibition and apoptotic induction effect of glycyrrhizin(GL) on human breast carcinoma(MCF-7) cells.The relationship between GL-induced apoptosis effect and intracellular Ca 2 concentration was also studied.Methods After MCF-7 cells were treated with different concentration of GL from 2.5 mmol/L to 12.5 mmol/L for 24 h,cell viability of proliferation was assessed by MTT assay.MCF-7 cells were treated with 5.0 mmol/L,7.5 mmol/L and 10.0 mmol/L GL for 24 h,and the rate of cell apoptosis was examined by terminal deoxynucleotide transferase mediated dUTP nick-end-labeling method and flow cytometry with Annexin V/propidium iodide fluorescent stain.7.5 mmol/L GL was used for 24 h and intracellular Ca 2 concentration was measured by Fure-2 fluorescein load method.Results With the concentration increase of GL from 5mmol/L to 12.5 mmol/L,the rate of proliferative inhibition increased significantly(P<0.01).The degree of the inhibition were in a dose-dependent fashion.IC 50 of the proliferative inhibition was 15.8 mmol/L.With GL of 7.5 mmol/L and 10.0 mmol/L,the rate of cell apoptosis increased significantly(P<0.05 and P<0.01).Intracellular Ca 2 concentration was lower evidently when treated with GL(P<0.05).Conclusion Glycyrrhizin has the proliferation inhibition and apoptosis induction effect on MCF-7 cell.The descent of intracellular Ca 2 level may be related to apoptosis induction effect of GL in MCF-7 cells.