人类组织激肽释放酶基因8在乳腺癌中的异常表达及其临床意义

目的：初步探讨人类组织激肽释放酶基因8（kallikrein gene8,KLK8）在乳腺疾病中的表达情况及其临床意义。方法：收集2008年10月至2009年7月,我院经病理证实的61例乳腺癌组织与相应癌旁组织标本以及26例乳腺良性肿瘤组织与相应瘤旁组织标本,分别通过实时PCR和Western印迹法在mRNA和蛋白质水平上检测KLK8在乳腺疾病中的表达情况。结果：KLK8在乳腺癌组织中的表达较其相应癌旁组织低（P〈0.0001）,在乳腺良性肿瘤中未发现该现象（P=0.424）,并且其在正常乳腺癌组织中的表达比乳腺良性肿瘤组织中表达低（P=0.001）。KLK8的表达与乳腺癌的肿瘤大小、淋巴结转移情况及临床分期存在相关。结论：KLK8在乳腺癌中呈低表达,可能是乳腺癌预后良好的一个肿瘤生物学标志。

Clinical significance of human KLK8 gene expression in breast cancer

Objective To investigate the expression and clinical significance of the human kallikrein gene 8（KLK8） in human breast diseases.Methods We collected 61cases of breast cancer tissue and corresponding neighboring normal tissue,26 cases of breast fibroadenoma tissue were also collected in order to compared with the breast cancer tissue.The expression of KLK8 in breast cancer and neigboring tissues,and breast fibroadenoma and neigboring tissues were evaluated by quantitative real-time RT-PCR（qRT） and Western blotting methods.Results The KLK8 was downregulated in breast cancer tissue and is not found the phenomenon was not noted in fibroadenoma.The downregulation of the KLK8 was associated to primary cancer size,nodal status and tumor staging.Conclusions The KLK8 is downregulated in breast cancer and it could be a favorable tumor biomarker showing a better prognosis.