Cyr61和VEGF在肝细胞癌及癌周肝硬化组织中的表达及相关性分析

目的分析高半胱氨酸蛋白16（Cyr61）和血管内皮生长因子（VEGF）在肝细胞癌、癌周肝硬化组织中的表达及相关性,探讨其在肝癌发生过程中的作用。方法采用免疫组化S-P法检测58例原发性肝细胞肝癌（HCC）及癌周肝硬化组织中Cyr61和VEGF蛋白的表达,并与其临床病理特征比较分析。结果Cyr61、VEGF在癌周肝硬化组织中的表达均高于HCC,差异有统计学意义（P〈0.01）;Cyr61、VEGF蛋白的表达均与肿瘤的转移密切相关（P〈0.05）;相关性检验提示Cyr61、VEGF在HCC组织、癌周肝硬化组织中呈正相关（r=0.444,P〈0.01）。结论Cyr61、VEGF异常表达与肝硬化肝癌的发展密切相关,可能在肝硬化向肝癌转化和肿瘤血管生成过程中发挥重要的作用,为肝癌的早期诊断提供了较为客观的参考指标。

Expression of Cyr61 and VEGF in hepatocellular carcinoma and hepatic cirrhosis tissues

Objective To analyse the expression and relations between Cyr61 and VEGF in human hepatocellular carcinoma（HCC） and hepatic cirrhosis tissues and their contribution to development of HCC.Methods Expressions of Cyr61 and VEGF in 58 cases of HCC and hepatic cirrhosis tissues were observed by immunohistochemistry S-P.The expression of protein was analysed and compared with clinical pathologic characters.Results The expressing of Cyr61 and VEGF was higher in hepatic cirrhosis than that in HCC tissues（P〈0.01）,and it was relative to metastasis of HCC（P〈0.05）.Cyr61 and VEGF had relativity in HCC and hepatic cirrhosis tissues by correlation test（r=0.444,P〈0.01）.Conclusion Abnormal expression of Cyr61 and VEGF is relative with the development of HCC and has significiant contribution in angiogenesis of HCC and transforming hepatic cirrhosis to HCC,which may provide a reference index to early diagnosis of HCC.