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Calcitonin gene-related peptide projection from the ventromedial thalamic nucleus to the insular cortex: a combined retrograde transport and immunocytochemical study
Authors:Sho¯  ichi Shimada, Sadao Shiosaka, Carmel J. Hillyard, Samia I. Girgis, Iain MacIntyre, Piers C. Emson,Masaya Tohyama
Affiliation:1. Department of Neuroanatomy, Institute of Higher Nervous Activity, Osaka University Medical School, 4-3-57 Nakanoshima, Kitaku, Osaka 530 Japan;2. Department of Chemical Pathology, University of London, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, London W12 OHS UK;3. MRC Neurochemical Pharmacological Unit, Medical Research Council Centre, Medical School, Hills Road, Cambridge CB2 2QH U.K.;1. Division of Oncology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri;2. Alvin J Siteman Cancer Center, Washington University in St. Louis, St. Louis, Missouri;1. Copenhagen Hearing and Balance Centre, Department of Oto-Rhino-Laryngology, Head and Neck Surgery, and Audiology, Copenhagen University Hospital – Rigshospitalet, Section 8047, Inge Lehmanns Vej 8, 2100, Copenhagen, Denmark;2. Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark;1. Department of Neurosurgery, Antwerp University Hospital, Edegem 2650, Belgium;2. Department of Translational Neuroscience, Faculty of Medicine and Health Science, University of Antwerp, Antwerp, Belgium;3. Department of Medicine, University of Cambridge, Addenbrooke''s Hospital, Cambridge, UK;1. Department of Cancer Medicine, Gustave Roussy, Villejuif, France;2. Department of Medical Oncology, National Cancer Centre Singapore, Duke–National University of Singapore Oncology Academic Clinical Programme, Singapore;3. Department of Oncology, University Hospital of Udine, Udine Italy;4. Pandit Bhagwat Dayal Sharma Postgraduate Institute of Medical Science, Rothak, India;5. Medical Oncology Department, University and Hospital Trust of Verona, Verona, Italy;6. Fourth Department of Medical Oncology and Clinical Trials Unit, Henry Dunant Hospital Center, Athens, Greece;7. Department of Respiratory Medicine, Maastricht University Medical Centre, GROW School for Oncology and Reproduction, Maastricht, The Netherlands
Abstract:We employed a highly sensitive combination method of retrograde tracing and immunohistochemistry to identify calcitonin gene-related peptide (CGRP)-containing fiber pathways in the rat from the ventrolateral part of the ventrolateral thalamic nucleus (vl-vl) and the caudal continuation to the insular cortex. Biotin-wheat germ agglutinin (B-WGA) injected into the insular cortex labeled numerous neurons in the vl-vl and the caudal continuation ipsilaterally; simultaneous staining with CGRP antiserum revealed that some of these neurons are CGRP positive.
Keywords:calcitonin gene-related peptide (CGRP)   projection   ventromedial thalamic nucleus   insular cortex   double-labeling method   rat
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