新生后给药地塞米松对大鼠肺形态发育的影响

目的:比较生后给予不同剂量地塞米松(dexamethasone,DEX)对大鼠肺组织形态发育的影响。方法:120只新生大鼠随机平均分为3组。小剂量DEX组:生后第5天起连续5天腹腔注射DEX0.2mg/(kg·d)。大剂量DEX组:生后第5天起连续5天腹腔注射DEX0.5mg/(kg·d)。生理盐水组:生后第5天起连续5天腹腔注射等量生理盐水。各组大鼠分别在生后第10、14、21、45天随机选取10只处死,分离肺组织,通过光镜观察,分析比较各组用药对肺形态发育的影响。结果:两DEX治疗组在生后各时间点每视野肺泡计数均低于生理盐水组(P﹤0.01),平均肺泡表面积均高于生理盐水组(P﹤0.05)。两DEX治疗组平均肺泡间隔厚度在生后第10、14天均低于生理盐水组(P﹤0.01),在生后第21天均高于生理盐水组(P﹤0.05)。结论:生后给DEX能显著促进肺部早期发育,但随着给药剂量的增多,肺泡化受阻现象逐渐明显,最终导致肺泡大而少的气肿样改变出现,其影响可持续至成年。

Effects of postnatal dexamethasone on rat lung morphogenesis

Objective:To compare the effects of different dosage of dexamethasone (DEX) on rat lung morphogenesis. Methods: Total of 120 newborn rats were divided into three groups randomly:a small dosage DEX group,a large dosage DEX group and a control group. On the 10th,14th,21st,45th days after birth,the histologic structures of 10 rat lungs in each group were observed with light microscope. Results:Under the light microscope,during the first 2 weeks,rat lungs in the two treatment groups had fewer alveolar numbers,l...