| 白藜芦醇诱导K562细胞凋亡过程中活性氧水平的改变 |
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| 引用本文： | 郝春燕,苏海翔,魏虎来,张哲文.白藜芦醇诱导K562细胞凋亡过程中活性氧水平的改变[J].中国临床药理学与治疗学,2004,9(10):1147-1149. |
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| 作者姓名： | 郝春燕 苏海翔 魏虎来 张哲文 |
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| 作者单位： | 1. 兰州医学院生物化学与分子生物学教研室,兰州,730000,甘肃
2. 兰州医学院生物化学与分子生物学教研室医学实验中心,兰州,730000,甘肃 |
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| 摘 要： | 目的：探讨白藜芦醇对K562细胞的凋亡诱导效应和对K562细胞内活性氧水平的影响。方法：应用噻唑蓝(MTr)比色法、光镜、DNA琼脂糖凝胶电泳和流式细胞术(FCM)检测细胞凋亡；FCM测定细胞内活性氧(ROS)水平。结果：白藜芦醇显著抑制K562细胞增殖，并呈现典型的凋亡形态学改变；DNA电泳可见梯状条带出现；FCM分析显示S期细胞数明显增多，出现S／G，期阻滞，ROS水平升高。结论：白藜芦醇诱导K562细胞凋亡与细胞内活性氧水平升高有关。
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| 关 键 词： | 白藜芦醇 白血病 K562 细胞凋亡 活性氧 |
| 文章编号： | 1009-2501(2004)10-1147-03 |
| 修稿时间： | 2004年7月31日 |
Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells |
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| HAO Chun Yan,SU Hai Xiang ,WEI Hu Lai ,ZHANG Zhe Wen.Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2004,9(10):1147-1149. |
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| Authors: | HAO Chun Yan SU Hai Xiang WEI Hu Lai ZHANG Zhe Wen |
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| Institution: | HAO Chun Yan,SU Hai Xiang 1,WEI Hu Lai 1,ZHANG Zhe Wen Department of Biochemistry and Molecular Biology,1 Laboratory Center for Medical Science,Lanzhou Medical College,Lanzhou 730000,Gansu,China |
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| Abstract: | AIM:To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level. METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM). RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G 2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS. CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production. |
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| Keywords: | resveratrol leukemia K562 apoptosis reactive oxygen species (ROS) |
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