| 腺病毒介导胸苷激酶基因治疗肝癌的实验研究 |
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| 引用本文： | 沈洪珍,袁学文,何小飞,李方和. 腺病毒介导胸苷激酶基因治疗肝癌的实验研究[J]. 咸宁医学院学报, 2000, 14(4): 250-252 |
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| 作者姓名： | 沈洪珍 袁学文 何小飞 李方和 |
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| 作者单位： | 广东省深圳市人民医院,深圳 |
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| 基金项目： | 湖北省教育厅重点资助课题! (编号 99A10 3) |
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| 摘 要： | 目的：观察腺病毒介导胸苷激酶基因联合抗病毒药物对人肝癌细胞（ＨｅｐＧ２）的杀伤作用。方法：利用腺病毒载体将单纯疱疹病毒胸苷激酶基因（ＨＳＶ－ＴＫ）导入体外培养的ＨｅｐＧ２中，再加入羟基无环鸟苷（ＧＣＶ），观察其对ＨｅｐＧ２细胞的杀伤效应。结果：在感染复数（ＭＯＩ）小于１００或ＧＣＶ浓度小于４００ｕｇ／ｍｌ范围内，单独应用载体或ＧＣＶ，对培养细胞的形态与存活率均无明显影响。当两者合并应用时，ＧＣＶ在
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| 关 键 词： | 腺病毒载体 胸苷激酶基因 肝癌 治疗 实验 |
| 文章编号： | 1008－0635(2000)04－0250－03 |
| 修稿时间： | 2000-09-18 |
An Experimental Study about Influence of Adenovirus-Mediated Transfer of the Herpes Simplex Virus Thymidine Kinase Gene and Prodrug on Hepatocarcinoma |
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| SHEN Hongzhen ,YUAN Xuewen ,He Xiaofei et al Shenzhen People's Hospital,Shenzhen First Affiliated Hospital,Xianning Medical College,Xianning $$$$. An Experimental Study about Influence of Adenovirus-Mediated Transfer of the Herpes Simplex Virus Thymidine Kinase Gene and Prodrug on Hepatocarcinoma[J]. Journal of Xianning Medical College, 2000, 14(4): 250-252 |
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| Authors: | SHEN Hongzhen YUAN Xuewen He Xiaofei et al Shenzhen People's Hospital Shenzhen First Affiliated Hospital Xianning Medical College Xianning $$$$ |
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| Affiliation: | SHEN Hongzhen 1,YUAN Xuewen 2,He Xiaofei 2 et al 1 Shenzhen People's Hospital,Shenzhen 518020 2 First Affiliated Hospital,Xianning Medical College,Xianning 437100$$$$ |
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| Abstract: | |
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| Keywords: | Adenovirus vector Thymidine kinase gene Ganciclovir HepG2 Apoptosis |
| 本文献已被 CNKI 维普 等数据库收录！ |
