CREM-1蛋白在食管鳞癌中的表达及临床意义

目的:研究环磷酸腺苷反应元件调节蛋白(Cyclic AMP response element modulator 1,CREM-1)在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中的表达情况,结合临床病理资料初步探讨CREM-1的表达水平与食管鳞癌发生转移和侵袭的相关性。方法:采用免疫组化二步法测定98例食管鳞癌癌标本CREM-1的表达水平。Western blot检测4组转移性与非转移性食管鳞癌新鲜标本中CREM-1的蛋白表达水平。结果:Western Blot结果显示CREM-1在转移性食管鳞癌中表达明显低于非转移性癌组织,与上皮标记物E-钙粘蛋白(E-cadherin)表达趋势一致。免疫组化结果显示CREM-1蛋白在转移性的食管鳞癌中低表达,在非转移性癌组织中高表达,差异有统计学意义(P<0.05)。统计学分析显示CREM-1的表达高低与与肿瘤分期相关(P<0.05)。生存分析显示CREM-1高表达的患者预后不良(P<0.05)。Cox模型多因素分析提示CREM-1是独立的预后指标(P<0.05)。结论:CREM-1在转移性食管鳞癌中低表达,可能参与调控食管鳞癌转移及侵袭的发生发展过程。

The expression and significance of CREM-1 in esophageal squamous cell carcinoma

Objective:To Investigate the expression of cyclic AMP response element modulator 1 protein in human esophageal squamous cell carcinoma(ESCC) and the relationship with esophageal cancer cell migration and invasion.Methods:The protein level of CREM-1 in fresh frozen esophageal carcinoma tissues was detected by Western blot.Immunohistochemical analysis was used to dectect the expression of CREM-1 in 98 esophageal carcinoma samples.Results:Western blot analysis showed expression of CREM-1 was significantly down-regulated in ESCC tissues with lymph nodes metastasis.Immunohistochemistry showed that the expression of CREM-1 was correlated with ESCC migration and TNM stage(P<0.05).The Kaplan-Meier survival curves showed that low CREM-1 expression was related to a poor survival with statistical significance(P<0.05).Multivariate Cox proportional hazards was regression analysis indicated that CREM-1 expression was an independent prognostic marker for esophageal carcinoma(P>0.05).Conclusions:The expression of CREM-1 was downregulated in metastasis ESCC tissues.These datas revealed that CREM-1 may play a role in the tumor matastasis and invasion of esophageal carcinoma.