SPE-UPLC-ELSD法测定柴胡中柴胡皂苷a、d的含量

目的：应用超高效液相色谱-蒸发光散射（UPLC—ELSD）检测柴胡中柴胡皂苷a、d的含量。方法：采用Waters Acquity UPLC BEH C18色谱柱（2．1mm×100mm,1．7μm）；以乙腈（A）-水（B）为流动相梯度洗脱（0－3min，43％A；3～9min，43％A→63％A）；流速：0．2mL．min-1：柱温：30℃；ELSD检测器漂移管温度为40℃，气体压力为40psi，测定柴胡中柴胡皂苷a、d的含量。结果：柴胡中柴胡皂苷a在浓度为64．2-385．2Dg·mL—1（r=0．9994）内线性范围良好：柴胡皂苷d在浓度为67．3～403．8μg·mL-1（r=0．9992）内线性范围良好。结论：该方法简便、灵敏、可靠、准确、重现洼好，可作为柴胡的质量控制方法。

Determination of Saikosaponin a, d in Radix Bupleuri by SPE-HPLC-ELSD

Objective： To determine saikosaponin a, d in Radix Bupleuri by SPE-HPLC-ELSD. Methods： The amounts of saikosaponin a, d in Radix Bupleuri were determined by SPE-UPLC-ELSD with a Waters Acquity UPLC BEH C18 column （2.1 mm×100mm,1.7μm） kept at 30℃, gradient mobile phase of acetonitrile （A）-water （B） 0-3 rain, 43% A; 3-9 rain, 43% A-63% A] at a flow rate of 0.2 mL.min-L The tube temperature of the detector was 40℃. The press of pure air was 40 psi. Result： The linear ranges for saikosaponin a and d were 64.2-385.2 μg·mL-1 （r=0.9994） and 67.3-403.8 μg·mL-1 （r=0.9992）, respectively. Conclusion： This method is easy, sensitive, reliable, accurate, reproducible and can be used as the quality control method of Radix Bupleuri.